MATERIALS AND METHODS

Materials

Sixteen patients with surgically resected SCC of the salivary gland were identified after searching an anonymized research database at Asan Medical Center between 2000 and 2018. Patient characteristics are listed in Tables 1 and 2. Eight patients (50%) had primary SCC in the salivary gland. The cases of primary SCC in the salivary gland had no past history of cancer and revealed no other concurrent lesions through imaging studies such as computed tomography (CT) and positron emission tomography. SCC that had been diagnosed at other sites and metastasized to the salivary gland were designated as metastatic SCC in this study. The remaining eight cases (50%) in this study had a history of SCC at other sites and were classified as metastatic SCC. The clinical parameters collected from the electronic medical records were age at onset, sex, smoking history, survival, and treatment history.

Histologic analysis

All cases were histologically reviewed by two pathologists (K.J.C. and U.J.) with standard immunohistochemistry tests for diagnosis. Pathologic review included the following features: degree of differentiation of SCC, comprehensive (general) histologic features of SCC, pattern of infiltration, lymphovascular invasion, presence of peritumoral lymphocytes, and desmoplastic reactions. The histologic features were compared between the primary tumor and metastatic SCC. Regarding the previous studies, the possible presence of squamous ductal metaplasia, ductal dysplasia, or a ductal carcinoma component was investigated to help elucidate the pathogenesis of primary SCC of the salivary gland [6,13].

Immunohistochemical analysis and special staining

Formalin-fixed paraffin-embedded tissue specimens from primary and metastatic SCCs of the salivary gland were subjected to mucicarmine staining and IHC staining for p16, p53, AR, GCDFP-15, and c-erbB2. IHC staining was performed using the autoimmunostainer BENCHMARK XT (Ventana Medical Systems, Tucson, AZ, USA) with the OptiView DAB IHC Detection Kit (Ventana Medical Systems) according to the manufacturer’s instructions using the reagents supplied with the kit. The antibodies used were as follows: mouse anti–GCDFP-15 (1:50, cat. MS1170A, clone 23A3, Neomarkers, Fremont, CA, USA), mouse anti-AR (1:100, cat. 200R-16, clone SP107, Cell Marque, Rocklin, CA, USA), mouse anti-p53 (1:1,000, cat. M7001, clone DO-7, Dako, Glostrup, Denmark), mouse antip16 INK4 (1:6, cat. 805-7413, clone E6H4, Ventana Medical Systems), and rabbit anti-c-erbB2 (1:8, cat. 790-4493, clone 4B5, Ventana Medical Systems). Tumor sections at a thickness of 4 μm were deparaffinized, and antigen retrieval was performed by incubation in EDTA buffer (cell conditioner #1) for 32 minutes. After inactivation of the endogenous peroxidases and rinsing with Tris buffer, the sections were incubated in diluted primary antibodies for 16 minutes at 37°C. Staining for mucicarmine was performed using the Mucicarmine Staining Kit (Ventana Medical Systems).

Immunostaining for p53 and AR was identified by a dark brown stain confined to the nucleus and was scored as positive regardless of proportion. For GCDFP-15 and p16, cytoplasmic staining was interpreted as an immunopositive response. C-erbB2 staining was evaluated using the American Society of Clinical Oncology (ASCO) 2018 system [10]. For mucicarmine staining, intracytoplasmic rose-red coloring was interpreted as a positive result.

RESULTS

Clinical features

Overall, 16 patients were diagnosed with SCC of the salivary gland after surgery. Eight cases were classified as primary SCC, and the remaining eight cases were metastatic SCC of the salivary gland. Primary SCC of the salivary gland was located in the parotid gland in five cases (63%) and the submandibular gland in three cases (38%). Metastasis to the salivary glands occurred in the parotid glands and originated from facial skin in three cases, external auditory canal in two cases, tongue in one case, eyelid in one case, and scalp in one case. Patients ranged in age from 45 to 85 years, with an average age of 64.8 years for patients with primary SCC and 69.1 years for those diagnosed with metastatic SCC. Ten men and six women were involved in the study.

The clinical characteristics of each patient diagnosed with primary SCC are summarized in Table 1. Six men and two women aged 58–77 years presented with a gradual increase in swelling of the parotid (5/8, 63%) or submandibular region (3/8, 38%). None of the participants had received radiation to the head and neck, and six patients had a history of smoking. Most salivary gland tumors were identified as a heterogeneous enhancing mass with a spiculated border and invasion of adjacent tissues on CT imaging except one case that presented as a benign tumor with a well-defined border. Resected tumors measured 2.8–6.3 cm in the greatest dimension. The pathological stage for the TNM Classification of Malignant Tumors (TNM) of primary SCC in the salivary gland was adjusted in each case according to the American Joint Committee on Cancer (AJCC) Staging Manual 8th edition (Supplementary Table S1). Four cases of primary SCC were assessed as pT3, while the other four cases of primary SCC were evaluated as pT2. Five cases of primary SCC had lymph node metastasis. Among them, three were classified as pN3b, while the remaining two were classified as pN2b and pN1. Of the eight patients, seven received adjuvant treatment in the form of radiotherapy or combined chemoradiotherapy after surgery. Four patients died after treatment, one experienced metastasis of the primary SCC to the lungs at one year, and one developed local recurrence upon follow-up.

The clinical characteristics of the patients diagnosed with metastatic SCC are summarized in Table 2. There were four men and four women aged 45–85 years, with all cases involving the parotid glands. Metastatic SCCs were identified by a palpable mass or on CT imaging during follow-up after initial diagnosis of SCC. Resected tumors measured 0.6–3 cm in the greatest dimension. Three patients had synchronous lymph node metastasis at the time of detection of metastatic SCC in the parotid gland.

Histologic features

Representative histological features of primary and metastatic SCC of the salivary gland are summarized in Table 3. The same general histologic features of SCC were observed in the primary and metastatic groups including keratinization, keratin pearls, intercellular bridges, and large nuclei with eosinophilic cytoplasm (Fig. 1A). Metastatic SCC tended to resemble primary SCC (Fig. 1B). The frequency of keratinization and keratin pearl formation was similar between the primary and metastatic groups. Extraparenchymal extension was present in eight and six cases of primary and metastatic SCC, respectively, and different growth patterns were observed at the tumor boundary. Primary SCC was characterized by significant desmoplastic reactions and lymphoplasma cell infiltration resulting in serrated pointed borders (Fig. 1C). Metastatic SCC exhibited less desmoplasia and inflammatory reactions and possessed bosselated smooth borders (Fig. 1D). Necrosis patterns also differed between the groups, showing spotty necrosis in primary SCC and a central pattern in metastatic SCC (Fig. 1E, F). Two cases (cases #5 and #7) in the primary group showed involvement of large ducts, continuing to the transitional or pseudostratified ductal epithelium (Fig. 1G), thought to be a metaplastic step. Case #7 also exhibited the coexistence of squamous and ductal carcinomas in the metastatic lymph nodes (Fig. 1H). Lymphovascular invasion and regional lymph node metastasis were observed in both groups. The frequency of lymphovascular invasion, lymph node metastasis, and bilateral lymph node metastasis was slightly higher in primary SCC (Table 3).

IHC findings

The IHC staining results for primary and metastatic SCC of the salivary gland are shown in Table 4. The expression rates of p53, p16, and GCDFP-15 did not differ significantly between the two groups, except for a slightly higher rate of p53 positivity in the primary SCC group. Mucicarmine staining was negative in all 16 cases, excluding high-grade mucoepidermoid carcinoma. One primary SCC (case #5) (13%) was positive for c-erbB2 (Fig. 2A). An immunopositive response for AR was observed in two primary SCCs (25%), of which one (case #6) exhibited AR expression within the primary tumor (Fig. 2B), while one (case #7) showed AR expression at ductal differentiation in lymph node metastasis (Fig. 2C). This case also demonstrated focal GCDFP-15 positivity (Fig. 2D).

Survival analysis

Overall survival (OS) was analyzed for primary and metastatic SCCs and compared with p16, p53, and AR IHC staining statuses of primary SCCs. Primary SCC did not exhibit significant differences in OS compared to metastatic SCCs (p=.992) (Supplementary Fig. S1A). When restricting the analysis to primary SCC only (n=8), there were no statistically significant differences in survival according to p16, p53, and AR statuses (Supplementary Fig. S1B-D). The p53-positive group exhibited a tendency toward shorter OS (p=.384) (Supplementary Fig. S1B).

DISCUSSION

SCC of the salivary gland is an uncommon occurrence, and most cases represent metastases from other head and neck sites [1,3,14]. There are few studies on primary SCC of the salivary gland [3,6,14], and a histologic comparison of primary and metastatic SCC has not been reported. The purpose of this study was to share helpful histologic features and diagnostic challenges between primary SCC and metastatic SCC of the salivary gland. Though previous studies researched variable kinds of salivary gland tumors including primary and metastatic tumors, the present series studied only SCCs of the salivary gland [6,14-16]. Therefore, primary SCCs of this study comprised a higher proportion (8 of 16 cases, 50%) among the total SCCs of the salivary gland retrieved over a 19-year period at one institution (Tables 1, 2). The histology of primary and metastatic SCCs was comparable even though some differences were observed. Primary SCCs exhibited a considerable desmoplastic reaction and peritumoral inflammation compared to metastatic SCCs, as well as a serrated margin and less central necrosis. However, these findings were non-specific, and distinguishing between the two groups was difficult based on histology alone.

Salivary duct involvement was observed in two primary SCCs, supporting the possible ductal origin of primary SCC of the salivary gland. Consistently, SCCs in situ arising from salivary ductal epithelium have been reported [6,13]. Flynn et al. [3] reported presence of squamous metaplasia within the ductal epithelium adjacent to invasive carcinoma in a fraction of primary SCC cases. One case in the present series exhibited a new finding of coexisting ductal and squamous carcinomas in a metastatic lymph node even though this phenomenon was not observed in the primary tumor. This ductal component was positive for AR and GCDFP-15 on IHC, consistent with salivary duct carcinoma, a predominantly apocrine tumor [7,17]. Although the other AR-positive case was pure SCC, AR-expressing areas contained lobules of non-keratinizing uniform cells, which is different from conventional SCC. AR expression in salivary SCC has not been reported [18-21]. These findings suggest a possible relationship between salivary duct carcinoma and SCC. A subset of primary SCCs of the salivary gland may be derived from extensive squamous differentiation of salivary duct carcinomas, whereas other cases may arise from squamous metaplasia of the salivary duct epithelium. While all metastatic SCCs involved the parotid gland, primary SCCs affected both parotid and submandibular glands. The parotid gland is the most commonly involved major gland in primary SCC [1,3,14], although many isolated cases of submandibular gland origin have been reported [4,5,16,22,23]. The location of primary SCC as well as the age and sex of the patients were similar to those reported for salivary duct carcinomas.

Previous radiation injury is a risk factor for salivary SCC development [4,5]. However, none of our patients had a history of radiation. Smoking is a risk factor for SCC of the head and neck. Six of the eight primary SCC patients were moderate smokers, supporting the role of smoking as a risk factor. The pathogenesis of primary SCC of the salivary gland remains to be determined.

An important issue in diagnosis of primary SCC of the salivary gland is exclusion of high-grade MEC. Mucin production suggests MEC or adenosquamous carcinoma arising in the salivary glands rather than primary SCC of the salivary gland. Taxy et al. [6] published controversial results regarding mucicarmine positivity in metastatic SCC of the submandibular gland and in one case of intraductal in situ SCC. The former case may have been metastatic MEC, while the latter case may have corresponded to early MEC. None of the primary or metastatic SCCs in this study exhibited intracytoplasmic mucin based on mucicarmine staining.

Primary SCC is a high-grade malignancy of the salivary gland that may require extensive excision with neck dissection and adjuvant radiotherapy [3]. A large series from the Surveillance, Epidemiology, and End Results database including 2,104 patients (not designated as primary) aged >85 years with a tumor size ≥4 cm identified extraparenchymal extension, cervical metastasis, and distant metastasis as independent prognostic factors [2]. Surgery resulted in better 5-year disease-specific survival rates compared to no surgery or radiation alone. In the present series, all patients were younger than 85 years, and survival outcomes after surgery with or without radiotherapy were variable. The small sample size was a limitation of our study, and no prognostic factors were determined from the IHC results. However, with respect to treatment, effects of anti-AR therapy in primary SCCs of the salivary gland are anticipated [19,23].

To accurately diagnose primary SCCs of the salivary gland, metastatic disease and high-grade MEC must be excluded through careful evaluation of relevant patient history and special staining. Although the pathogenesis of salivary SCC remains unknown, the present histology and IHC results suggest a possible association with salivary duct carcinoma.

Supplementary Materials

Fig. 1.

Histologic features of primary squamous cell carcinoma (SCC) and metastatic SCC of the salivary gland. (A, B) Keratinization, keratin pearls, and intercellular bridges are observed in primary SCC (A, case #8; inset, ×100) and metastatic SCC (B, case #15; left, original SCC; right, metastatic SCC). (C, D) Representative tumor border and tumoral microenvironment in primary and metastatic SCC. Primary SCC shows serrated sharp borders (C, case #5; inset, peritumoral inflammation and desmoplasia), while metastatic SCC shows a bosselated smooth boundary (D, case #14; inset, ×200). (E, F) Tumor necrosis is irregularly distributed in primary SCC (E, case #8; inset, ×200) and centrally located in metastatic SCC (case #16). (G) Primary SCC continuing to transitional or pseudostratified ductal epithelium (case #5). (H) The transitional area of salivary duct carcinoma in SCC is observed in a metastatic lymph node (case #7).

Fig. 2.

Results of immunohistochemical staining in primary squamous cell carcinoma (SCC) and metastatic SCC. (A) Immunopositive reaction to c-erbB2 in primary SCC (case #5). (B, C) Immunopositive response to androgen receptor staining in the salivary duct carcinoma component of the primary tumor (B, case #6) and metastatic tumor (case #7) in primary SCC. (D) Focal positivity for gross cystic disease fluid protein 15 in a metastatic tumor of primary SCC in a lymph node (case #7).

REFERENCES

• 1. Batsakis JG. Primary squamous cell carcinomas of major salivary glands. Ann Otol Rhinol Laryngol 1983; 92(1 Pt 1):97-8. ArticlePubMedPDF
• 2. Chen MM, Roman SA, Sosa JA, Judson BL. Prognostic factors for squamous cell cancer of the parotid gland: an analysis of 2104 patients. Head Neck 2015; 37: 1-7. ArticlePubMedPDF
• 3. Flynn MB, Maguire S, Martinez S, Tesmer T. Primary squamous cell carcinoma of the parotid gland: the importance of correct histological diagnosis. Ann Surg Oncol 1999; 6: 768-70. ArticlePubMedPDF
• 4. Manvikar V, Ramulu S, Ravishanker ST, Chakravarthy C. Squamous cell carcinoma of submandibular salivary gland: a rare case report. J Oral Maxillofac Pathol 2014; 18: 299-302. ArticlePubMedPMC
• 5. Panchbhai AS. Primary squamous cell carcinoma of salivary gland: report of a rare case. J Cancer Res Ther 2015; 11: 664.
• 6. Taxy JB. Squamous carcinoma in a major salivary gland: a review of the diagnostic considerations. Arch Pathol Lab Med 2001; 125: 740-5. PubMed
• 7. Williams L, Thompson LD, Seethala RR, et al. Salivary duct carcinoma: the predominance of apocrine morphology, prevalence of histologic variants, and androgen receptor expression. Am J Surg Pathol 2015; 39: 705-13. PubMed
• 8. Alsahafi E, Begg K, Amelio I, et al. Clinical update on head and neck cancer: molecular biology and ongoing challenges. Cell Death Dis 2019; 10: 540.ArticlePubMedPMCPDF
• 9. Zhou G, Liu Z, Myers JN. TP53 mutations in head and neck squamous cell carcinoma and their impact on disease progression and treatment response. J Cell Biochem 2016; 117: 2682-92. ArticlePubMedPMC
• 10. Wolff AC, Hammond ME, Allison KH, Harvey BE, McShane LM, Dowsett M. HER2 testing in breast cancer: American Society of Clinical Oncology/College of American Pathologists clinical practice guideline focused update summary. J Oncol Pract 2018; 14: 437-41. ArticlePubMed
• 11. Glisson B, Colevas AD, Haddad R, et al. HER2 expression in salivary gland carcinomas: dependence on histological subtype. Clin Cancer Res 2004; 10: 944-6. PubMed
• 12. Xu B, Wang L, Borsu L, et al. A proportion of primary squamous cell carcinomas of the parotid gland harbour high-risk human papillomavirus. Histopathology 2016; 69: 921-9. ArticlePubMedPMCPDF
• 13. Gallego L, Junquera L, Calvo N, Fuente E, Rosado P. Bilateral carcinoma in situ of Wharton's duct after chronic obstructive sialadenitis: inflammation as the cause of malignancy? Ann Otol Rhinol Laryngol 2012; 121: 296-300. ArticlePubMedPDF
• 14. Franzen A, Lieder A, Guenzel T, Buchali A. The heterogenicity of parotid gland squamous cell carcinoma: a study of 49 patients. In Vivo 2019; 33: 2001-6. ArticlePubMedPMC
• 15. Bron LP, Traynor SJ, McNeil EB, O'Brien CJ. Primary and metastatic cancer of the parotid: comparison of clinical behavior in 232 cases. Laryngoscope 2003; 113: 1070-5. ArticlePubMed
• 16. Kulkarni AA, Thakur SS. Primary squamous cell carcinoma of submandibular salivary gland with sialo-cutaneous fistula: a rare case report. J Clin Diagn Res 2015; 9: PD03-5. Article
• 17. Kapadia SB, Barnes L. Expression of androgen receptor, gross cystic disease fluid protein, and CD44 in salivary duct carcinoma. Mod Pathol 1998; 11: 1033-8. PubMed
• 18. Can NT, Lingen MW, Mashek H, et al. Expression of hormone receptors and HER-2 in benign and malignant salivary gland tumors. Head Neck Pathol 2018; 12: 95-104. ArticlePubMedPDF
• 19. Dalin MG, Watson PA, Ho AL, Morris LG. Androgen receptor signaling in salivary gland cancer. Cancers (Basel) 2017; 9: 17.ArticlePubMedPMC
• 20. Nasser SM, Faquin WC, Dayal Y. Expression of androgen, estrogen, and progesterone receptors in salivary gland tumors: frequent expression of androgen receptor in a subset of malignant salivary gland tumors. Am J Clin Pathol 2003; 119: 801-6. ArticlePubMedPDF
• 21. Udager AM, Chiosea SI. Salivary duct carcinoma: an update on morphologic mimics and diagnostic use of androgen receptor immunohistochemistry. Head Neck Pathol 2017; 11: 288-94. ArticlePubMedPMCPDF
• 22. Agarwal M, Agarwal L, Saxena R. Primary squamous cell carcinoma of submandibular salivary gland: a case report. J Clin Diagn Res 2017; 11: XD01-2. Article
• 23. Gaikwad RV, Kumaraswamy SV, Keerthi R. Primary squamous cell carcinoma of the submandibular salivary gland: a rare entity. J Maxillofac Oral Surg 2015; 14(Suppl 1):57-9. ArticlePDF

Figure & Data

References

Citations

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