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Automated immunohistochemical assessment ability to evaluate estrogen and progesterone receptor status compared with quantitative reverse transcription-polymerase chain reaction in breast carcinoma patients
Taesung Jeon, Aeree Kim, Chungyeul Kim
J Pathol Transl Med. 2021;55(1):33-42.   Published online December 3, 2020
DOI: https://doi.org/10.4132/jptm.2020.09.29
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  • 4 Web of Science
  • 4 Crossref
AbstractAbstract PDF
Background
This study aimed to investigate the capability of an automated immunohistochemical (IHC) evaluation of hormonal receptor status in breast cancer patients compared to a well-validated quantitative reverse transcription–polymerase chain reaction (RT-qPCR) method.
Methods
This study included 93 invasive breast carcinoma cases that had both standard IHC assay and Oncotype Dx assay results. The same paraffin blocks on which Oncotype Dx assay had been performed were selected. Estrogen receptor (ER) and progesterone receptor (PR) receptor status were evaluated through IHC stains using SP1 monoclonal antibody for ER, and 1E2 monoclonal antibody for PR. All ER and PR immunostained slides were scanned, and invasive tumor areas were marked. Using the QuantCenter image analyzer provided by 3DHISTECH, IHC staining of hormone receptors was measured and converted to histochemical scores (H scores). Pearson correlation coefficients were calculated between Oncotype Dx hormone receptor scores and H scores, and between Oncotype Dx scores and Allred scores.
Results
H scores measured by an automated imaging system showed high concordance with RT-qPCR scores. ER concordance was 98.9% (92/93), and PR concordance was 91.4% (85/93). The correlation magnitude between automated H scores and RT-qPCR scores was high and comparable to those of Allred scores (for ER, 0.51 vs. 0.37 [p=.121], for PR, 0.70 vs. 0.72 [p=.39]).
Conclusions
Automated H scores showed a high concordance with quantitative mRNA expression levels measured by RT-qPCR.

Citations

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  • Vision Transformers for Breast Cancer Human Epidermal Growth Factor Receptor 2 Expression Staging without Immunohistochemical Staining
    Gelan Ayana, Eonjin Lee, Se-woon Choe
    The American Journal of Pathology.2024; 194(3): 402.     CrossRef
  • Marker assessments in ER‐positive breast cancers: old markers, new applications?
    Joshua J X Li, Gary M Tse
    Histopathology.2023; 82(2): 218.     CrossRef
  • The Story of the Magee Equations: The Ultimate in Applied Immunohistochemistry
    Rohit Bhargava, David J. Dabbs
    Applied Immunohistochemistry & Molecular Morphology.2023; 31(7): 490.     CrossRef
  • Dose-Dependent Relationship between Protection of Thioacetamide-Induced Acute Liver Injury and Hyperammonemia and Concentration of Lactobacillus salivarius Li01 in Mice
    Pengcheng Lou, Yangfan Shen, Aoxiang Zhuge, Longxian Lv, Xueling Zhu, Yin Yuan, Liya Yang, Kaicen Wang, Bo Li, Lanjuan Li, Joanna B. Goldberg
    Microbiology Spectrum.2021;[Epub]     CrossRef
Morphological and Functional Changes in the Thyroid Follicles of the Aged Murine and Humans
Junguee Lee, Shinae Yi, Yea Eun Kang, Hyeon-Woo Kim, Kyong Hye Joung, Hae Joung Sul, Koon Soon Kim, Minho Shong
J Pathol Transl Med. 2016;50(6):426-435.   Published online October 14, 2016
DOI: https://doi.org/10.4132/jptm.2016.07.19
  • 11,769 View
  • 360 Download
  • 27 Web of Science
  • 30 Crossref
AbstractAbstract PDF
Background
Although both thyroid histology and serum concentrations of hormones are known to change with age, only a few reports exist on the relationship between the age-related structural and functional changes of the thyroid follicles in both mice and humans. Our objectives were to investigate age-related histological changes of the thyroid follicles and to determine whether these morphological changes were associated with the functional activity of the follicles.
Methods
The thyroid glands of mice at 18 weeks and at 6, 15, and 30 months of age were histologically examined, and the serum levels of thyroid hormones were measured in 11-week-old and 20-month-old mice. Samples of human thyroid tissue from 10 women over 70 years old and 10 women between 30 and 50 years of age were analyzed in conjunction with serum thyroid hormone level.
Results
The histological and functional changes observed in the thyroid follicles of aged mice and women were as follows: variable sizing and enlargement of the follicles; increased irregularity of follicles; Sanderson’s polsters in the wall of large follicles; a large thyroglobulin (Tg) globule or numerous small fragmented Tg globules in follicular lumens; oncocytic change in follicular cells; and markedly dilated follicles empty of colloid. Serum T3 levels in 20-month-old mice and humans were unremarkable.
Conclusions
Thyroid follicles of aged mice and women show characteristic morphological changes, such as cystic atrophy, empty colloid, and Tg globules.

Citations

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  • Histological evidence of hypothyroidism in mice chronically exposed to conventional farming
    Nádia Coelho, Ricardo Camarinho, Patrícia Garcia, Armindo S. Rodrigues
    Environmental Toxicology and Pharmacology.2024; 106: 104387.     CrossRef
  • Thyroid‐on‐a‐Chip: An Organoid Platform for In Vitro Assessment of Endocrine Disruption
    Daniel J. Carvalho, Anna M. Kip, Mírian Romitti, Marta Nazzari, Andreas Tegel, Matthias Stich, Christian Krause, Florian Caiment, Sabine Costagliola, Lorenzo Moroni, Stefan Giselbrecht
    Advanced Healthcare Materials.2023;[Epub]     CrossRef
  • Serious lesions in Green turtles (Chelonia mydas) afflicted by fatal Spirorchiidiasis found stranded in south and southeastern Brazil
    Hassan Jerdy, Bruna Barreto, Max Werneck, Rachel Ann Hauser-Davis, Paula Baldassin, Patrick Gabriel, Aline Luize de Moraes Souza, Maria Aparecida da Silva, Aline Felix, Rachel Ribeiro Rodrigues, Mariah Bianchi, Carla Barbosa, Gessica Gomes Vieira, Lara Ri
    International Journal for Parasitology: Parasites and Wildlife.2023; 20: 73.     CrossRef
  • 基于光学相干层析技术的甲状腺乳头状癌图像识别
    李增鸣 Li Zengming, 赵潮 Zhao Chao, 张旭 Zhang Xu, 毛伟征 Mao Weizheng, 赵航 Zhao Hang, 史晓凤 Shi Xiaofeng, 马君 Ma Jun
    Laser & Optoelectronics Progress.2023; 60(4): 0417002.     CrossRef
  • Differentiating Thyroid Follicular Adenoma from Follicular Carcinoma via G-Protein Coupled Receptor-Associated Sorting Protein 1 (GASP-1)
    Yuan Rong, Cesar Torres-Luna, George Tuszynski, Richard Siderits, Frank N. Chang
    Cancers.2023; 15(13): 3404.     CrossRef
  • The effects of glyphosate-based herbicide on the hypothalamic-pituitary thyroid axis are tissue-specific and dependent on age exposure
    Jeane Maria Oliveira, Jamilli Zenzeluk, Paula Bargi-Souza, Raphael Escorsim Szawka, Marco Aurelio Romano, Renata Marino Romano
    Environmental Pollution.2023; 334: 122216.     CrossRef
  • Borealin/CDCA8 deficiency alters thyroid development and results in papillary tumor-like structures
    Hortense Didier-Mathon, Athanasia Stoupa, Dulanjalee Kariyawasam, Sonny Yde, Beatrix Cochant-Priollet, Lionel Groussin, Frédéric Sébag, Nicolas Cagnard, Patrick Nitschke, Dominique Luton, Michel Polak, Aurore Carré
    Frontiers in Endocrinology.2023;[Epub]     CrossRef
  • Arsenic-Induced Thyroid Hormonal Alterations and Their Putative Influence on Ovarian Follicles in Balb/c Mice
    Nandheeswari K, Jayapradha P, Sree Vaishnavi Nalla, Itishree Dubey, Sapana Kushwaha
    Biological Trace Element Research.2023;[Epub]     CrossRef
  • Notch signaling in thyrocytes is essential for adult thyroid function and mammalian homeostasis
    Lluc Mosteiro, Thi Thu Thao Nguyen, Simona Hankeova, Daniel Alvarez-Sierra, Mike Reichelt, Shannon M. Vandriel, Zijuan Lai, Feroza K. Choudhury, Dewakar Sangaraju, Binita M. Kamath, Alexis Scherl, Ricardo Pujol-Borrell, Robert Piskol, Christian W. Siebel
    Nature Metabolism.2023; 5(12): 2094.     CrossRef
  • Toward Systems-Level Metabolic Analysis in Endocrine Disorders and Cancer
    Aliya Lakhani, Da Hyun Kang, Yea Eun Kang, Junyoung O. Park
    Endocrinology and Metabolism.2023; 38(6): 619.     CrossRef
  • Сравнение морфологических изменений щитовидной железы крыс послевлияния разных видов алиментарной депривации
    Р. В. Янко
    Biological Journal of Armenia.2022; : 24.     CrossRef
  • Zinc Depletion Inhibits the Synthesis and Secretion of Thyroglobulin by Inducing Endoplasmic Reticulum Stress in PCCL3 Thyroid Cells
    Kisang Kwon, Eun-Ryeong Lee, Kyung-Hee Kang, Tae-Sik Hwang, Seung-Whan Kim, Hyewon Park, O-Yu Kwon
    International Journal of Biology and Biomedical Engineering.2022; 16: 290.     CrossRef
  • Metimazol ile Oluşturulan Sıçan Hipotiroidi Modelinde Kognitif Fonksiyonlar, Anksiyete ve Depresyon Benzeri Davranışların Değerlendirilmesi: Pilot Çalışma
    Oğuzhan Ekin EFE, Selda EMRE AYDINGÖZ, Karl Michael LUX, Eda ÖZER, Ayşegül SÜZER, Meral TUNCER
    Uludağ Üniversitesi Tıp Fakültesi Dergisi.2022; 48(2): 147.     CrossRef
  • Loss of thyroid gland circadian PER2 rhythmicity in aged mice and its potential association with thyroid cancer development
    Junguee Lee, Hae Joung Sul, Hyunsu Choi, Dong Hyun Oh, Minho Shong
    Cell Death & Disease.2022;[Epub]     CrossRef
  • HISTOTOPOGRAPHIC AND STRUCTURAL FEATURES OF CANINE THYROID GLAND FOLLICLES UNDER SINGLE DYNAMIC MOTOR LOADS
    Andrey V. Bezdenezhnykh, Sofia S. Kolodezhnaya, Daria E. Nazarova, Anna P. Bavrina
    Morphological newsletter.2022; 30(4): 22.     CrossRef
  • Thyroid hormone receptor β sumoylation is required for thyrotropin regulation and thyroid hormone production
    Sujie Ke, Yan-Yun Liu, Rajendiran Karthikraj, Kurunthachalam Kannan, Jingjing Jiang, Kiyomi Abe, Anna Milanesi, Gregory A. Brent
    JCI Insight.2021;[Epub]     CrossRef
  • Thyroid Gland Alterations in Old-Aged Wistar Rats: A Comprehensive Stereological, Ultrastructural, Hormonal, and Gene Expression Study
    Marko Miler, Vladimir Ajdžanović, Jasmina Živanović, Jelena Marković Filipović, Branka Šošić-Jurjević, Verica Milošević
    Microscopy and Microanalysis.2021; 27(2): 437.     CrossRef
  • Age-dependent effects on radiation-induced carcinogenesis in the rat thyroid
    Mutsumi Matsuu-Matsuyama, Kazuko Shichijo, Katsuya Matsuda, Nariaki Fujimoto, Hisayoshi Kondo, Shiro Miura, Tomomi Kurashige, Yuji Nagayama, Masahiro Nakashima
    Scientific Reports.2021;[Epub]     CrossRef
  • Relevant dose of the environmental contaminant, tributyltin, promotes histomorphological changes in the thyroid gland of male rats
    Paula Rodrigues-Pereira, Sofia Macedo, Tiago Bordeira Gaspar, Sule Canberk, Samia Selmi-Ruby, Valdemar Máximo, Paula Soares, Leandro Miranda-Alves
    Molecular and Cellular Endocrinology.2020; 502: 110677.     CrossRef
  • Morphological and functional changes in neonatally X-irradiated thyroid gland in rats
    Nariaki Fujimoto, Mutsumi Matsuu-Matsuyama, Masahiro Nakashima
    Endocrine Journal.2020; 67(2): 231.     CrossRef
  • Optical coherence tomography for thyroid pathology: 3D analysis of tissue microstructure
    Iulian Emil Tampu, Michaela Maintz, Daniela Koller, Kenth Johansson, Oliver Gimm, Arrigo Capitanio, Anders Eklund, Neda Haj-Hosseini
    Biomedical Optics Express.2020; 11(8): 4130.     CrossRef
  • Thyroid surgery in the elderly
    Nicole Ruszkay, David Goldenberg, Guy Slonimsky
    Operative Techniques in Otolaryngology-Head and Neck Surgery.2020; 31(3): 206.     CrossRef
  • Liver X Receptor β Related to Tumor Progression and Ribosome Gene Expression in Papillary Thyroid Cancer
    Seonhyang Jeong, In-Kyu Kim, Hyunji Kim, Moon Jung Choi, Jandee Lee, Young Suk Jo
    Endocrinology and Metabolism.2020; 35(3): 656.     CrossRef
  • A historical excursus of diagnostic methods for Hashimoto thyroiditis and Graves' disease
    Maria Trovato
    Gazzetta Medica Italiana Archivio per le Scienze Mediche.2020;[Epub]     CrossRef
  • Cross‐sectional comparison of health‐span phenotypes in young versus geriatric marmosets
    Corinna N. Ross, Jessica Adams, Olga Gonzalez, Edward Dick, Luis Giavedoni, Vida L. Hodara, Kimberley Phillips, Anna D Rigodanzo, Balakuntalam Kasinath, Suzette D. Tardif
    American Journal of Primatology.2019;[Epub]     CrossRef
  • Effects of nonylphenols on embryonic development and metamorphosis of Xenopus laevis: FETAX and amphibian metamorphosis toxicity test (OECD TG231)
    Yang Xu, Sun Jung Park, Myung Chan Gye
    Environmental Research.2019; 174: 14.     CrossRef
  • Basal Autophagy Deficiency Causes Thyroid Follicular Epithelial Cell Death in Mice
    Tomomi Kurashige, Yasuyo Nakajima, Mika Shimamura, Mutsumi Matsuyama, Masanobu Yamada, Masahiro Nakashima, Yuji Nagayama
    Endocrinology.2019; 160(9): 2085.     CrossRef
  • Aging Is Associated with Low Thyroid State and Organ-Specific Sensitivity to Thyroxine
    Helena Rakov, Meri De Angelis, Kostja Renko, Georg Sebastian Hönes, Denise Zwanziger, Lars Christian Moeller, Karl-Werner Schramm, Dagmar Führer
    Thyroid.2019; 29(12): 1723.     CrossRef
  • High-fat, simple-carbohydrate diet intake induces hypothalamic–pituitary–thyroid axis dysregulation in C57BL/6J male mice
    Nagaraj Banavara Swarnalatha, Neena Roy, Mahesh Manjunath Gouda, Rajeish Moger, Asha Abraham
    Applied Physiology, Nutrition, and Metabolism.2018; 43(4): 371.     CrossRef
  • Biomarker Sensitivity to Vehicle Exhaust in Experimentally Exposed European Starlings
    Michelle A. North, Jaime Rodriguez-Estival, Judit E. G. Smits
    Environmental Science & Technology.2017; 51(22): 13427.     CrossRef
A Study of Bcl-2 Oncoprotein Expression in Endometrial Carcinoma Correlated with Hormone Receptor Status.
Young Im Han, Hye Jin Lee, Ji Yeon Lee, Sun Kyung Lee
Korean J Pathol. 1996;30(5):408-416.
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AbstractAbstract PDF
Bcl-2 is a proto-oncogene initially described in follicular lymphoma, associated with chromosomal translocation(14;18). Recent studies have shown the presence of Bcl-2 in nonhematolymphoid tissue, especially in hormonally responsive tissue. The endometrium is an attractive model for studying the hormone dependent regulation of Bcl-2 expression. We have studied the immunoreactivity of Bcl-2 oncoprotein in relation to the immunoreactivity of estrogen receptors(ER) and progesterone receptors(PR) by immunohistochemistry in 52 human endometrial carcinomas, according to nuclear grade. The results obtained are summarized as followings, 1) Immunohistochemical grade of Bcl-2 showed a significant inverse correlation with nuclear grade. 2) Immunohistochemical grades of ER and PR also showed a significant inverse correlation with nuclear grade, and were well correlated with each other. 3) Immunohistochemical grades of Bcl-2 and hormone receptors showed a strongly significant correlation. On the basis of the above results, we suggest that Bcl-2 expression may be under hormone dependent control and that it can be used in prognosis and choice of hormonal therapy in the presence of hormone receptor.
E-Cadherin Expression in Breast Carcinoma: Correlation with Tumor Grade and Hormone Receptor.
Haeng Ji Kang, Chan Pil Park, Chan Kum Park
Korean J Pathol. 1997;31(11):1172-1179.
  • 1,714 View
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AbstractAbstract
E-cadherin (E-CD), a Ca2+ -dependent adhesion molecule, plays a major role in the maintenance of intercellular junctions in normal epithelial cells in most organs. Recently, a correlation has been observed between a loss of E-CD and increased invasiveness of neoplastic cells. In this study, E-CD expression in the breast carcinoma was investigated using monoclonal antibody, anti-E-CD by immunohistochemical method. Expression of E-CD were evaluated in 57 breast carcinomas and correlated with their tumor grade, lymph node involvement, and hormonal receptor status. Histological types included in this study were 54 invasive ductal carcinomas (IDCs) of otherwise not specified and 3 invasive lobular carcinomas. Cases of histologic grade I IDC were 6, grade II 30, and grade III 18. Of 54 IDCs 39 (72.2%) showed moderate to strong linear staining at the cell borders regardless of their histologic grade, status of lymph node metastasis, and status of hormone receptor. Staining intensity of E-CD was reduced in 54 cases (83%) of IDC when compared with that of normal or benign breast lesions (P<0.01). All seven cases of intraductal carcinoma, which were included in 54 IDCs showed one or two grade reduced expression of E-CD than that of infiltrative lesions. Three invasive lobular carcinomas showed strong (1 case), moderate (1 case), and negative reactivity (1 case). The data indicated that loss of E-CD expression is a crucial event in the development of breast carcinoma.
Comparison between Immunohistochemical Stains and Serum Hormone Level on Pituitary Adenomas.
Hye Seung Han, Yeon Lim Suh
Korean J Pathol. 1998;32(2):88-93.
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AbstractAbstract PDF
The current classification of pituitary adenomas is based on cell type, largely ascertained by immunohistochemistry and electron microscopy. In an application of immunohistochemistry, pathologists have some problems in judging the results. An immunostaining result does not always correspond with a serum hormone level. It is also difficult to determine the nature of a tumor when a few cells are positive. We performed the immunohistochemical stains on 34 pituitary adenomas using polyclonal antibodies to six pituitary hormones [PRL (prolactin), GH (growth hormone), ACTH (adrenocorticotropic hormone), FSH (follicle-stimulating hormone), LH (luteinizing hormone), TSH (thyroid-stimulating hormone)] and compared with serum hormone level. The serum hormone level was increased in 14 cases (41.2%) of PRL, 7 cases (20.6%) of PRL & GH, 6 pleurihormonal cases (17.6%), 4 nonfunctioning cases (11.8%), 2 cases (5.9%) of FSH, and 1 case (2.9%) of GH. The most common immunohistochemical type of pituitary adenoma was 10 prolactinoma cases (38.5%), followed by 7 pleurihormonal cases (26.9%), 4 null cell cases (15.4%), 3 cases of mixed PRL & GH (11.5%), 1 case of ACTH (3.8%) and 1 FSH & LH case (3.8%). The corresponding rates of the serum hormone level and immunostaining results were 94.1% in GH, 88.9% in TSH, 85.7% in LH, 82.4% in ACTH, 66.7% FSH, and 61.8% in PRL. In the immunostaining for FSH, 12 cases showed less than 5% positivity and most of them exhibited the normal serum hormone level. In conclusion, the most common elevated serum hormone and immunohistochemical type of pituitary adenoma was prolactinoma. The corresponding rate of the serum hormone level and immunostaining result was the highest in GH cell adenoma and was the lowest in prolactinoma. The cells showing less than 5% positivity seem to be entrapped normal cells.
Expression of Androgen Receptor, bcl-2 Protein and Rb Protein in Breast Cancers as Related to Prognostic Factors.
Hyun Deuk Cho, Young Sik Kim, Insun Kim
Korean J Pathol. 1998;32(6):443-452.
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AbstractAbstract
Breast cancer is the most common malignancy of women in industrialized countries and the third in Korean women. There have been a lot of studies on biologic behaviors as well as on the prognostic factors of the breast cancer. In this study, the expression of hormone receptors (estrogen receptor ; ER, progesterone receptor ; PR, and androgen receptor ; AR), bcl-2 and Rb proteins were immunostained on 60 infiltrating ductal carcinomas of the breast. The results were evaluated in relation with the age, histologic grade, tumor size, and status of lymph node metastasis. The expression of ER and PR had a significant inverse correlation with the histologic grade (p<0.001). The expression of AR was significantly correlated with the expression of ER (p=0.039) and PR (p=0.009), but not with other factors such as age, histologic grade, tumor size, and status of lymph node metastasis. There was no relationship between bcl-2 protein expresssion and other prognostic factors, except with the expression of ER. Rb protein expression had a significant positive relationship with PR (p=0.05) and the degree of positivity was correlated with histologic grade (p=0.002). In conclusion, this result suggests that expression of AR is well correlated with ER and PR. bcl-2 has no prognostic value. The significance of Rb protein expression remained to be studied.
Assessment of DNA Ploidy, Estrogen and Progesterone Recetor Status and Her-2/neu Oneoprotein Expression in Breast Carcinoma by Image Analysis.
Ae Ree Kim, In Sun Kim, Kap No Lee
Korean J Pathol. 1994;28(3):246-259.
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AbstractAbstract
In 41 cases of breast cancers, the aneuploidy measured by Image Analyzer was compared with that of flow cytometric analysis, and estrogen and progesterone receptor(ER/PR) and Her-2/neu oncoprotein were immunohistochemically stained and measured by Image Analyzer. In ER/PR, the positive nuclear area(PNA, %) was measured, and in Her-2/neu, the content of oncoprotein was expressed as pg/cell. To assess the usefulness of these parameters as a prognostic factor, the author evaluated the results in relation with tumor size, nuclear grade and lymph node metastasis. The obtained results are summarized as follows: 1) The detection rate (90%) of aneuploidy by image analysis was higher than that (70%) of flow cytometric analysis. The concordance rate of both method was 80%. 2) The positivity of ER was 73% and PR was 34%, and the high PNA of ER and PR was related with high nuclear grade. There was an inverse correlation of the ER PNA with tumor size and PR PNA with negative lymph node. 3) Her-2/neu oncoprotein overexpression was found in only 2 cases and another two showed borderline overexpression. All four cases had DNA tetraploidy. From the above results, it was concluded that the image analyzer could be used in DNA analysis and in quantitation of immunostained ER/PR and Her-2/neu oncoprotein, providing the important information in the management of the breast cancer patients.

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