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Sung and Kim: Appendiceal actinomycosis mimicking appendiceal tumor, appendicitis or inflammatory bowel disease

Abstract

Appendiceal actinomycosis is very rare and its diagnosis is often difficult even in surgically resected specimens. Here we report two cases of appendiceal actinomycosis confirmed by pathologic examination of surgically resected specimens. Characteristic histologic features included transmural chronic inflammation with Crohn-like lymphoid aggregates and polypoid mucosal protrusion into cecal lumen through fibrous expansion of the submucosa. Chronic active inflammation involved the mucosa of the appendix and cecum around the appendiceal orifice. Crohn’s disease with predominant cecal involvement and inflammatory pseudotumor were considered as differential diagnoses. Careful examination revealed a few actinomycotic colonies in the mucosa, confirming the diagnosis. A high index of suspicion with awareness of the characteristic histologic features might prompt careful inspection for the actinomycotic colonies, leading to the appropriate diagnosis of this rare disease.

Actinomyces are gram-positive anaerobic bacteria, one of the commensals of human mucous membrane [1]. They can cause chronic inflammation when normal mucous membrane is damaged due to various causes such as abdominal surgery, bowel preparation, or trauma [2]. The most common species in human is Actinomycetes israelii and it is known to usually involve cervicofacial, thoracic, and abdominal areas [3,4]. The appendix is the most preferred organ in the abdominal area [4,5]. Appendiceal actinomycosis is very rare and presents with nonspecific symptoms, which can mimic several other diseases including appendiceal malignancy, acute appendicitis, or inflammatory bowel disease. Because treatment is different depending on the diagnosis, unnecessary surgery can be avoided if malignancy is excluded. Therefore, there have been several studies attempting to diagnose this condition with specific radiologic or endoscopic features [1,4,6,7]. However, these radiologic, endoscopic, and clinical features are not diagnostic, and the final diagnosis of actinomycosis usually requires pathologic confirmation after exploratory surgery [5]. Until recently, there have been limited reports in the literature describing the pathologic features of appendiceal actinomycosis [1,5,7-13]. If the causative organism is easily identifiable in biopsy material, the diagnosis can be rendered without surgery. However, a pathologic diagnosis is often difficult even on the resected specimen because this disease can mimic Crohn’s disease or inflammatory pseudotumor and the causative organisms are sometimes very hard to find. Here, we report two patients with appendiceal actinomycosis to help pathologists recognize and diagnose this rare disease.

CASE REPORT

Case 1

A 61-year-old woman presented with a mass-like lesion on imaging studies during health screening without any specific symptom. She had been diagnosed with breast cancer 9 years ago. Laboratory tests revealed mild leukocytosis (11.1×102/μL) and increased C-reactive protein (3.41 mg/dL). Abdominal contrast-enhanced computed tomography (CT) revealed appendicolith, periappendiceal fat infiltration with prominent wall thickening, and arterial enhancement in the appendiceal base (Fig. 1A). No mural thickening or contrast enhancement was found in the small intestine or other parts of the colon. Based on these findings, appendicitis with peri-appendiceal abscess or other inflammatory mass was suspected, but a primary appendiceal neoplasm could not be completely excluded. Colonofiberscopy (CFS) showed cecal contraction with multiple small polyps and scars in the large intestine. Intestinal tuberculosis was added to the list of differential diagnoses. Colonoscopic biopsy showed a focal active colitis pattern only and no actinomycotic colonies were found (data not shown).
Ileocecal resection was performed for histologic confirmation. Upon surgery, a mass-like lesion was observed in the appendiceal orifice and regional ileocolic lymph nodes were enlarged. On gross examination, the base of the appendiceal wall was markedly thickened and fibrotic (Fig. 1B). The appendiceal lumen was filled with fecal materials. On microscopy, the most striking features under low magnification were transmural chronic inflammation with Crohn-like lymphoid aggregates and marked mural thickening (Fig. 2A). The thickened appendiceal and peri-appendiceal cecal wall showed multiple foci of mixed chronic inflammatory cell infiltration and fibrosis reminiscent of inflammatory pseudotumor (Fig. 2C). The mucosa showed chronic active inflammation, including cryptitis, crypt abscess, crypt distortion, and lymphoplasmacytic infiltration in the lamina propria (Fig. 2D). Fibrous thickening of the subserosa and reactive regional lymph node hyperplasia were observed but there was no neutrophilic infiltration in the proper muscle. A few colonies of filamentous micro-organism of about 150 µm were found within the mucosa as well as in the appendiceal lumen (Fig. 2E), and the filamentous nature of the colonies were better appreciated on periodic acid–Schiff staining (Fig. 2F). The patient was diagnosed with appendiceal actinomycosis and was discharged without any complications. No additional antibiotics were taken, and until 6 months after surgery, there were no complications or recurrence of actinomycosis.

Case 2

A previously healthy 45-year-old man was incidentally found to have a submucosal mass in the periappendiceal orifice during routine CFS screening. An approximately 1.0 cm-sized hyperemic mucosal bulging was observed around the appendiceal orifice (Fig. 1C). Like case No. 1, the causative organisms were not found in the colonoscopic biopsy specimen. Contrast-enhanced abdominal CT showed intraluminal calcification and enhancing wall thickening in the appendix. Physical examination and laboratory investigation were unremarkable. Based on these findings, both chronic appendicitis and appendiceal malignancy were considered.
The patient underwent ileocecal resection. On gross examination, mucosal bulging (about 1.0 cm in the greatest dimension) was observed in the appendiceal orifice (Fig. 1D). The entire appendiceal wall was fibrotic and the periappendiceal soft tissue was rough and hemorrhagic. A few actinomycotic colonies with surrounding active inflammation were observed in the mucosa. In contrast to case No. 1, additional gross examination and step sectioning were required for identification of the organisms. Prominent transmural reactive lymphoid hyperplasia was also present mainly in the appendix. Mucosal bulging, which looked like a mass lesion on CFS, turned out to be reactive fibroadipose tissue hypertrophy due to chronic inflammation (Fig. 2B). The patient was diagnosed with appendiceal actinoymycosis and discharged with oral antibiotics (amoxicillin) for 2 months. Ten months after the surgery, the patient had no other complications or recurrence of actinomycosis.

DISCUSSION

The purpose of this report is to further define the pathologic features of appendiceal actinomycosis so that an accurate pathologic diagnosis can be made. Both cases had localized chronic transmural inflammation with scattered Crohn-like lymphoid aggregates or follicles across the base of the appendix and the cecum around the appendiceal orifice. In addition, there was reactive fibroadipose tissue hypertrophy that appeared as a bulging mass in the appendiceal orifice. Actinomycotic colonies, which are critical for the diagnosis, were relatively hard to find and only a few colonies were observed in the superficial lamina propria and appendiceal lumen. The appendiceal tip had fibrosis similar to chronic appendicitis, and active inflammation was rarely observed. Although a confirmatory diagnosis through colonoscopic biopsy is usually limited, awareness of these pathologic features might lead to an accurate diagnosis of this rare inflammatory mass-like lesion.
To date, there have been a few reports in the literature describing appendiceal actinomycosis, and their clinicopathologic features are summarized in Table 1 [1,5,7-13]. Among the 10 cases, six cases were initially diagnosed with appendicitis, while atypical inflammatory condition was suspected in four cases, and one case was suspected to have a malignant tumor on imaging studies. In our series, both patients were asymptomatic, and a wide range of preoperative differential diagnoses including neoplasm, chronic appendicitis, inflammatory mass-like lesion, and tuberculosis were considered. Radiologically, the appendiceal actinomycosis can mimic a malignant neoplasm because it can show contrast enhancement and infiltration into the surrounding tissue. Although these are actually granulation tissue and fibrotic reaction associated with actinomycosis [6], complete exclusion of malignancy is often impossible. Furthermore, goblet cell adenocarcinoma of the appendix may frequently mimic an inflammatory lesion on abdominal CT [14], and tumor cells may not reach the appendiceal orifice in many of them. For these reasons, surgical exploration is frequently required for confirmatory diagnosis.
In pathological perspective, Crohn’s disease is histologically quite similar to the actinomycosis in that chronic transmural inflammation is evident. In such cases, it is more suggestive of Crohn’s disease when the lesion is not limited to the appendix and histologic features such as patchy lymphoplasmacytic infiltration, non-caseating granulomas, fissure or fistula are observed in bowel segments other than the appendix. Thus, integration of colonoscopic findings and pathologic features is required to exclude Crohn’s disease in most cases.
While acute appendicitis should be treated by surgical removal of the appendix [15,16], asymptomatic appendiceal actinomycosis as in our cases might be amenable to medical treatment with antibiotics [5]. Surgery is reserved for patients who fail to respond to antibiotic therapy, or for those with complicated disease as a therapeutic adjunct [4,5,8]. When patients do not show a progressive clinical course, and malignancy is not in the top list of radiologic differential diagnosis, antibiotic therapy might be carefully tried in the presence of microscopic proof of actinomycotic colonies. If patients respond to medical treatment, surveillance endoscopic biopsy along with radiologic test can be used afterwards. If not, pathologic confirmation after surgical resection is required [4,5,7].
To try medical treatment in a patient with suspected appendiceal actinomycosis, detection of the organisms in colonoscopic material is essential. There is a report on an appendiceal actinomycosis case diagnosed by endoscopic biopsy [17]. In the report, the patient had a past history of prior surgical resection for colon adenocarcinoma, and follow up colonoscopic biopsy on a nodular lesion in the anastomosis site revealed actinomycotic colonies. However, in general, diagnostic actinomycotic colonies are seldom detected in biopsy material like the present series. Regarding the diagnostic sensitivity for the actinomycotic colonies, there is no consensus upon how many pieces of biopsy are recommended for detection of the causative organisms. In general, a greater number of biopsies would lead to a better diagnostic yield. However, abdominal actinomycosis cannot be easily diagnosed without surgical intervention and colonoscopic approach is often limited in terms of specimen size and accessibility to the lesion especially for that in the appendix. Thus, ultrasound or CT guided aspiration or biopsy, or explorative laparotomy may be a more realistic diagnostic option [5,9-11]. Furthermore, if there is a past history of previous surgical procedure, bowel preparation, trauma, or prolonged use of intrauterine devices, more aggressive preoperative diagnostic approach might be needed.
In conclusion, integration of clinical, colonoscopic, and radiological features with pathologic findings is necessary to diagnose appendiceal actinomycosis. Awareness of these characteristic clinicopathologic features might lead to suspect and accurately diagnose this uncommon, but clinically important disease. Because the causative organisms are often very hard to find, serial sectioning is advised in colonoscopic biopsy material and extensive sampling is advised in surgically resected specimen.

Notes

Ethics Statement

Formal written informed consents were waived by the Institutional Review Board of Asan Medical Center (2019-1213).

Author contributions

Conceptualization: JK. Data curation: YNS, JK. Investigation: YNS, JK. Writing—original draft: YNS, JK. Approval of final manuscript: all authors.

Conflicts of Interest

The authors declare that they have no potential conflicts of interest.

Funding

No funding to declare.

Fig. 1.
Radiologic, colonoscopic, and gross features. (A) Contrast-enhanced computed tomography of case No. 1 shows appendicolith, fat infiltration with prominent wall thickening with contrast enhancement in the appendiceal base (arrow). (B) The resected appendix shows marked mural thickening, dilated appendiceal lumen and central fecalith. (C) Colonoscopic image of case No. 2 shows an approximately 10 mm-sized hyperemic mucosal bulging around the appendiceal orifice. (D) Cut surface of the resected specimen shows mucosal bulging (about 1.0cm in great dimension) on the orifice of appendix (arrow).
jptm-2020-05-17f1.jpg
Fig. 2.
Representative microscopic pictures. (A) Chronic transmural inflammation with scattered lymphoid follicles or aggregates are observed. (B) The bulging mucosa in colonoscopy is hypertrophic fibroadipose tissue that is covered by inflamed mucosa. (C) One of the fibroinflammatory foci shows marked lymphoplasmacytic infiltration with fibrosis that is reminiscent of inflammatory pseudotumor. (D) The inflamed mucosa of the appendix shows marked lymphoplasmacytosis, crypt abscess, and crypt distortion. (E) An actinomycotic colony is surrounded by mixed inflammatory cell infiltrates. (F) The filamentous nature is better demonstrated by Periodic acid–Schiff staining.
jptm-2020-05-17f2.jpg
Table 1.
Clinicopathologic features of reported cases of appendiceal actinomycosis
Case Study Age (yr)/Sex Symptom Radiologic or colonoscopic finding Initial diagnosis Pathologic feature Treatment
1 Atalaia-Martins et al. (2018) [8] 66/F Abdominal pain Subepithelial luminal protrusion in the region of the appendiceal orifice with a whitish liquid material discharge Uncertain Polymorphic cell infiltrate and erosion with appendiceal fibrosis Right hemicolectomy
2 Gomez-Torres et al. (2017) [5] 39/M Abdominal pain, fever, nausea, and vomiting Non compressible enlarged cecal appendix (58 mm) with peri-appendiceal edema Appendicitis Chronic fistulized appendicitis with transmural lymphoid infiltration Appendectomy
3 Liu et al. (2016) [7] 53/M Abdominal pain, fever Distended appendix (9 mm) with surrounding mesenteric stranding Appendicitis Lymphoid hyperplasia and chronic inflammatory cells in muscularis propria and serosa Appendectomy with IV/oral antibiotics (6 months)
4 Liu et al. (2016) [7] 54/F Abdominal pain, fever Markedly thickened appendix (18 mm) with periappendiceal fat stranding Appendicitis Suppurative granulomatous inflammation Preoperative antibiotics and drainage with elective appendectomy
5 Ng et al. (2014) [12] 19/F Abdominal pain, nausea, and vomiting Markedly swollen appendix (2.3 cm) with a calcified appendicolith Uncertain Extensive chronic inflammation and eosinophilic infiltration within the wall of appendix Ileocecectomy
6 Karakus et al. (2014) [13] 14/M Abdominal pain and vomiting Increased (10 mm) thickness of the appendix with nonperistaltic ileocecal region on ultrasonography Appendicitis Vermiform appendix with neutrophilic infiltration Appendectomy
7 Lee et al. (2010) [1] 50/F Incidental finding (routine screening) Well defined mass (2 cm) at the origin of appendix Appendiceal neoplasm of mucosal origin Localized abscess formation of the appendiceal wall Appendectomy
8 Nissotakis et al. (2008) [9] 31/M Abdominal pain No sign of appendicitis on X-ray and ultrasonography Appendicitis Transmural inflammatory cell infiltrate with lymphoid hyperplasia and fibrosis Appendectomy with oral antibiotics (6 months)
9 Karagulle et al. (2008) [10] 51/F Abdominal pain, fever and vomiting 3 × 2-cm-sized enhancing mass near cecum Appendicitis Chronic active inflammation around sulfur granules Appendectomy with IV/oral antibiotics (3 months)
10 Koren et al. (2002) [11] 83/F Abdominal pain Lobular mass (5 m) attached to the cecum Uncertain Dense inflammatory cell infiltration within muscularis and fibro- purulent reaction over the serosa Right hemicolectomy with IV/oral antibiotics (6 months)

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