Journal of Pathology and Translational Medicine

Original Article

ERG Immunohistochemistry as an Endothelial Marker for Assessing Lymphovascular Invasion: Sehun Kim, Hyung Kyu Park, Ho Young Jung, So-Young Lee, Kyueng-Whan Min, Wook Youn Kim, Hye Seung Han, Wan Seop Kim, Tae Sook Hwang, So Dug Lim; Korean J Pathol. 2013;47(4):355-364. Published online August 26, 2013; DOI: https://doi.org/10.4132/KoreanJPathol.2013.47.4.355

10,153 View
64 Download
24 Crossref

Background

ERG, a member of the ETS family of transcription factors, is a highly specific endothelial marker. We investigated whether the use of ERG immunostaining can help pathologists detect lymphovascular invasion (LVI) and decrease interobserver variability in LVI diagnosis.

Methods

Fifteen cases of surgically resected colorectal cancers with hepatic metastasis were selected and the most representative sections for LVI detection were immunostained with ERG, CD31, and D2-40. Eight pathologists independently evaluated LVI status on hematoxylin and eosin (H&E) and the corresponding immunostained sections and then convened for a consensus meeting. The results were analyzed by kappa (κ) statistics.

Results

The average rate of LVI positivity was observed in 43% with H&E only, 10% with CD31, 29% with D2-40, and 16% with ERG. Agreement among pathologists was fair for H&E only (κ=0.27), D2-40 (κ=0.21), ERG (κ=0.23), and was moderate for CD31 (κ=0.55). Consensus revealed that ERG nuclear immunoreactivity showed better visual contrast of LVI detection than the other staining, with improved agreement and LVI detection rate (κ=0.65, LVI positivity rate 80%).

Conclusions

The present study demonstrated a superiority with ERG immunostaining and indicated that ERG is a promising panendothelial marker that might help pathologists increase LVI detection and decrease interobserver variability in LVI diagnosis.

Citations

Citations to this article as recorded by

Exercise mitigates flow recirculation and activates metabolic transducer SCD1 to catalyze vascular protective metabolites
Susana Cavallero, Mehrdad Roustaei, Sandro Satta, Jae Min Cho, Henry Phan, Kyung In Baek, Ana M. Blázquez-Medela, Sheila Gonzalez-Ramos, Khoa Vu, Seul-Ki Park, Tomohiro Yokota, Jennifer Sumner, Julia J. Mack, Curt D. Sigmund, Srinivasa T. Reddy, Rongsong
Science Advances.2024;[Epub] CrossRef
Cardiac delivery of modified mRNA using lipid nanoparticles: Cellular targets and biodistribution after intramyocardial administration
M.C.I. Labonia, M. Estapé Senti, P.H. van der Kraak, M.A.D. Brans, I. Dokter, T.J. Streef, A.M. Smits, A.K. Deshantri, S.C.A. de Jager, R.M. Schiffelers, J.P.G. Sluijter, P. Vader
Journal of Controlled Release.2024; 369: 734. CrossRef
Endometrial cancer: predictors and oncological safety of tumor tissue manipulation
Raquel Quintana-Bertó, Pablo Padilla-Iserte, Víctor Lago, Carmen Tauste, Berta Díaz-Feijoo, Silvia Cabrera, Reyes Oliver-Pérez, Pluvio J. Coronado, María Belén Martín-Salamanca, Manuel Pantoja-Garrido, Josefa Marcos-Sanmartin, Eduardo Cazorla, Cristina Lo
Clinical and Translational Oncology.2023; 26(5): 1098. CrossRef
Universal method for the isolation of microvessels from frozen brain tissue: A proof-of-concept multiomic investigation of the neurovasculature
Marina Wakid, Daniel Almeida, Zahia Aouabed, Reza Rahimian, Maria Antonietta Davoli, Volodymyr Yerko, Elena Leonova-Erko, Vincent Richard, René Zahedi, Christoph Borchers, Gustavo Turecki, Naguib Mechawar
Brain, Behavior, & Immunity - Health.2023; 34: 100684. CrossRef
The Usefulness of Elastin Staining to Detect Vascular Invasion in Cancer
Jeffrey Gonzalez, Hisham F. Bahmad, Stephanie Ocejo, Alvaro Abreu, Meagan Popp, Samantha Gogola, Vielka Fernandez, Monica Recine, Robert Poppiti
International Journal of Molecular Sciences.2023; 24(20): 15264. CrossRef
Single‐nucleus transcriptome analysis reveals disease‐ and regeneration‐associated endothelial cells in white matter vascular dementia
Daniel N. Mitroi, Min Tian, Riki Kawaguchi, William E. Lowry, S. Thomas Carmichael
Journal of Cellular and Molecular Medicine.2022; 26(11): 3183. CrossRef
Gene of the month: ERG
Dorinda Mullen, Klaudia Nowak, Runjan Chetty
Journal of Clinical Pathology.2022; 75(9): 577. CrossRef
Oncological safety of hysteroscopy in endometrial cancer
Raquel Quintana-Bertó, Pablo Padilla-Iserte, Antonio Gil-Moreno, Reyes Oliver-Pérez, Pluvio J. Coronado, María Belén Martín-Salamanca, Manuel Pantoja-Garrido, Cristina Lorenzo, Eduardo Cazorla, Juan Gilabert-Estellés, Lourdes Sánchez, Fernando Roldán-Riva
International Journal of Gynecologic Cancer.2022; 32(11): 1395. CrossRef
Upregulated PD-1 signaling antagonizes glomerular health in aged kidneys and disease
Jeffrey W. Pippin, Natalya Kaverina, Yuliang Wang, Diana G. Eng, Yuting Zeng, Uyen Tran, Carol J. Loretz, Anthony Chang, Shreeram Akilesh, Chetan Poudel, Hannah S. Perry, Christopher O’Connor, Joshua C. Vaughan, Markus Bitzer, Oliver Wessely, Stuart J. Sh
Journal of Clinical Investigation.2022;[Epub] CrossRef
Primary Soft Tissue Sarcoma of the Heart: An Emerging Chapter in Cardio-Oncology
Pietro Scicchitano, Maria Chiara Sergi, Matteo Cameli, Marcelo H. Miglioranza, Marco Matteo Ciccone, Marica Gentile, Camillo Porta, Marco Tucci
Biomedicines.2021; 9(7): 774. CrossRef
Analysis on the healing of gastrointestinal ulceration by using Hemospray
Christoph R. Werner, Lena Brücklmeier, Thomas Kratt, Nisar P. Malek, Bence Sipos, Dörte Wichmann, Martin Götz
Scientific Reports.2021;[Epub] CrossRef
Immunohistochemistry for diagnosis and prognosis of breast cancer: a review
T. Bonacho, F. Rodrigues, J. Liberal
Biotechnic & Histochemistry.2020; 95(2): 71. CrossRef
TMEM100 is a key factor for specification of lymphatic endothelial progenitors
Eun-Hye Moon, Yong Hwan Kim, Phuong-Nhung Vu, Hyunjin Yoo, Kwonho Hong, Young Jae Lee, S. Paul Oh
Angiogenesis.2020; 23(3): 339. CrossRef
Spectral photon-counting CT imaging of colorectal peritoneal metastases: initial experience in rats
Arnaud Thivolet, Salim Si-Mohamed, Pierre-Emmanuel Bonnot, Christophe Blanchet, Vahan Képénékian, Loïc Boussel, Philippe Douek, Pascal Rousset
Scientific Reports.2020;[Epub] CrossRef
Diagnostic and reporting issues of preneoplastic polyps of the large intestine with early carcinoma
Dale C. Snover
Annals of Diagnostic Pathology.2019; 39: 1. CrossRef
Feasibility of Endoscopic Resection in Early Gastric Cancer with Lymphovascular Invasion
Jeung Hui Pyo, Hyuk Lee, Yang Won Min, Byung-Hoon Min, Jun Haeng Lee, Kyoung-Mee Kim, Hyeseung Kim, Kyunga Kim, Jae J. Kim
Annals of Surgical Oncology.2019; 26(2): 449. CrossRef
Lymphovascular Invasion and the Decision for Postmastectomy Radiation Therapy: A Cautionary Case Report
J. Arthur Jensen, Rania Bakkar, Michele Burnison, Armando E. Giuliano
Plastic and Reconstructive Surgery - Global Open.2019; 7(2): e2115. CrossRef
Utility of ERG Immunohistochemistry for Evaluation of Lymphovascular Invasion in Testicular Germ Cell Tumors: A Retrospective Pilot Study
Aaron M. Udager, Jonathan B. McHugh, Todd M. Morgan, Daniel E. Spratt, Arul M. Chinnaiyan, Rohit Mehra
Applied Immunohistochemistry & Molecular Morphology.2019; 27(5): 392. CrossRef
An interesting observation from our experiences with circulating tumour cells
Kok H. Lim, Yong W. Chua, Tony K.H. Lim, Angela M. Takano
Lung Cancer.2017; 108: 247. CrossRef
Differences in Prevalence of Lymphovascular Invasion among Early Gastric Cancers between Korea and Japan
Sun-Young Lee, Naohisa Yoshida, Osamu Dohi, Sang Pyo Lee, Daisuke Ichikawa, Jeong Hwan Kim, In-Kyung Sung, Hyung Seok Park, Eigo Otsuji, Yoshito Itoh, Chan Sup Shim, Hye Seung Han, Mitsuo Kishimoto, Yuji Naito
Gut and Liver.2017; 11(3): 383. CrossRef
Tumor Budding Is an Independent Adverse Prognostic Factor in Pancreatic Ductal Adenocarcinoma
Kate O’Connor, Hector H. Li-Chang, Steven E. Kalloger, Renata D. Peixoto, Douglas L. Webber, David A. Owen, David K. Driman, Richard Kirsch, Stefano Serra, Charles H. Scudamore, Daniel J. Renouf, David F. Schaeffer
American Journal of Surgical Pathology.2015; 39(4): 472. CrossRef
P2X7Receptor Expression in Coexistence of Papillary Thyroid Carcinoma with Hashimoto's Thyroiditis
Ji Hyun Kwon, Eun Sook Nam, Hyung Sik Shin, Seong Jin Cho, Hye Rim Park, Mi Jung Kwon
Korean Journal of Pathology.2014; 48(1): 30. CrossRef
Evaluation of colon cancer histomorphology: a comparison between formalin and PAXgene tissue fixation by an international ring trial
Sibylle Gündisch, Julia Slotta-Huspenina, Paolo Verderio, Chiara Maura Ciniselli, Sara Pizzamiglio, Christina Schott, Enken Drecoll, Christian Viertler, Kurt Zatloukal, Marcel Kap, Peter Riegman, Irene Esposito, Katja Specht, Gregor Babaryka, Martin Assla
Virchows Archiv.2014; 465(5): 509. CrossRef
Tumor Sprouting in Papillary Thyroid Carcinoma Is Correlated with Lymph Node Metastasis and Recurrence
Eunjung Lee, Wonkyung Jung, Jeong-Soo Woo, Jae Bok Lee, Bong Kyung Shin, Han Kyeom Kim, Aeree Kim, Baek-hui Kim
Korean Journal of Pathology.2014; 48(2): 117. CrossRef

Case Report

Sclerosing Angiomatoid Nodular Transformation (SANT) in Spleen: A Case Report.: Hyun Jung Lee, Song Yi Choi, Song Mei Huang, Ji Young Sul, Jin Man Kim; Korean J Pathol. 2011;45(1):111-114.; DOI: https://doi.org/10.4132/KoreanJPathol.2011.45.1.111

3,821 View
50 Download
4 Crossref

Abstract PDF

Sclerosing angiomatoid nodular transformation (SANT) of spleen is a rare inflammatory tumor-like vascular lesion composed of angiomatoid nodules in a fibrosclerotic background. We report herein on a case of SANT in the spleen with its pathologic features, and review the related literature. A 50-year-old woman presented with mild left upper quadrant discomfort and tenderness and she showed a 6 cm-sized solitary splenic mass on computed tomography. She underwent laparoscopic splenectomy. Grossly, the spleen showed a well circumscribed round-shaped solid mass with multinodular hemorrhagic surfaces. Microscopically, the mass consisted of multiple angiomatoid nodules surrounded by collagen bundles with fibroblasts and a lymphoplasma cell infiltration. Immunohistochemically, the cells of the angiomatoid nodules were positive for CD31, CD30, CD34, alpha-smooth muscle actin, and VWF-VIII, but they were negative for CD8, anaplastic lymphoma kinase protein, and D2-40. The patient has been under close follow-up without recurrence.

Citations

Citations to this article as recorded by

Sclerosing angiomatoid nodular transformation of the spleen presenting rapid growth after adrenalectomy: Report of a case
Yasuo Nagai, Daisuke Satoh, Hiroyoshi Matsukawa, Shigehiro Shiozaki
International Journal of Surgery Case Reports.2017; 30: 108. CrossRef
A Case of Sclerosing Angiomatoid Nodular Transformation of the Spleen: Spoke Wheel Pattern on Computed Tomography
Joo Han Park, Sun Hyuk Hwang, Ki Chan Kim, Si Yeon Kim, Ga Won Song, Jae Ho Han, Joon Seong Park
Korean Journal of Medicine.2014; 86(3): 357. CrossRef
Differentiation of an Unclear Splenic Lesion in a Patient With Cholangiocarcinoma
Constantin Lapa, Ulrich Steger, Christian O. Ritter, Vanessa Wild, Ken Herrmann
Clinical Nuclear Medicine.2014; 39(5): 470. CrossRef
Sclerosing Angiomatoid Nodular Transformation of the Spleen: CT and MRI Features With Pathologic Correlation
Rachel B. Lewis, Grant E. Lattin, Meenakshi Nandedkar, Nadine S. Aguilera
American Journal of Roentgenology.2013; 200(4): W353. CrossRef

Original Articles

Correlation between Tumor Angiogenesis (Microvessel Density), Metastasis and Tumor Cell Proliferation in Colorectal Carcinomas.: Young Chae Chu, Joon Mee Kim; Korean J Pathol. 1997;31(6):517-526.

1,602 View
14 Download

Abstract PDF: Tumor angiogenesis has been shown to be associated with metastatic potentials in breast, lung and prostatic carcinomas. The relation between tumor angiogenesis and metastatic potentials in colorectal cancer has not been established to date. We analysed 66 selected patients with colorectal carcinomas (37 with and 29 without nodal metastases) for the microvessel density, tumor proliferation activity, and the clinicopathologic parameters including size, stage, histologic grade, growth pattern, presence of angioinvasion, perineural invasion and lymph node metastasis. For evaluation of microvessel density and tumor proliferative activity, the primary tumors were immunohistochemically stained for CD31 and PCNA. The mean microvessel counts (MVC) per 200X field were 99.27+/-23.28 and 131.35+/-31.48 in node-negative and node-positive patients, respectively. The PCNA index was 39.41+/-5.63% and 56.60+/-7.09% in node-negative and node-positive patients, respectively. MVC and PCNA index were higher in tumors with nodal metastasis (p=0.002, p<0.001), and also correlated each other (sr=0.33, p=0.007). Higher microvessel counts were seen in tumors with advanced stage (p=0.016). Tumor proliferation activity assessed by PCNA immunostaining was significantly higher in tumors with advanced stage, perineural invasion, angioinvasion, poor differentiation and larger size. From these results, MVC and PCNA index in colorectal carcinomas are assumed to be valuable prognostic parameters. Thus assessment of tumor angiogenesis and tumor cell proliferation in colorectal carcinomas may be helpful for the patients in need of aggressive therapy.

Infantile Hemangioendothelioma of the Liver: A histological and immunohistochemicalstudy of 4 cases.: Mee Yon Cho, Sun Hee Sung, Soon Hee Jung, Chanil Park; Korean J Pathol. 1995;29(2):145-151.

1,603 View
17 Download

Abstract PDF: A pathological study was performed on four cases of infantile hemangioendothelioma of the liver. All the patients were between the age of 1 -5 months and the tumors were typical hemangio-endotheliomas, type 1. The tumors were composed basically of two components; the endothelial cell proliferation and the myxoid matrix. The endothelial cells were cytologically innocuous and formed vascular channels of varying sizes and shapes from capillary to sinusoidal and cavernous vessels. Fibrosis of the matrix, albeit not a major component of the tumor, was found particularly near the center. Immunohistochemically, CD31 was expressed strongly in almost all endothelial cells, in contrast to the stain for von Willebrand factor which was only focally and weakly positive. Alpha-fetoprotein was expressed in hepatocytes within the tumor or in hepatocytes around the tumor. Intratumoral bile duct structures were located mainly at peripheral portion. The results indicated that the type I infantile hemangioendothelioma is a tumor of endothelial cells and myxoid stroma, and that the endothelial cells undergo gradual maturation to form sinusoidal and cavernous vessels in accordance with gradual fibrosis of the myxoid stroma.

First
Prev
Page of 1
Next
Last

Search