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The Korean Journal of Pathology 2007;41(1): 15-20.
Immunohistochemical Profile of Acute Cellular Rejection in Renal Allograft.
Jongha Park, Seung Woon Byun, Eunsil Yu, Su Kil Park, Duck Jong Han, Yong Mee Cho
1Department of Nephrology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Korea.
2Department of Pathology, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Korea. youngcho@amc.seoul.kr
3Department of Surgery, University of Ulsan College of Medicine, Asan Medical Center, Seoul 138-736, Korea.
ABSTRACT
BACKGROUND: We wanted to find an adjunctive marker(s) in renal allograft biopsies for predicting acute cellular rejection (ACR), and so the expression patterns of immune-related molecules were compared between ACR, borderline ACR and non-ACR cases. METHODS: The expression patterns of Fas ligand (FasL), HLA-DR, granzyme B, caspase-3, CD56, interferon stimulated growth factor-3 (ISGF-3), and CD53 were assessed via immunohistochemical study in 75 allograft biopsies from patients with ACR (n=19), borderline ACR (n=22), and non-ACR (n=34). RESULTS: Compared to those of the non-ACR group, the ACR group revealed an elevated number of FasL positive interstitial inflammatory cells, HLA-DR positive tubular inflammatory cells, cytoplasmic caspase-3 positive tubular epithelial cells, granzyme B positive interstitial mononuclear inflammatory cells and CD53 positive interstitial inflammatory cells. The expression patterns of the borderline ACR group were similar to those of non-ACR group, except for the intensity of FasL in the tubular epithelial cells. CONCLUSIONS: Immunohistochemical investigations of the adjunctive markers FasL, HLA-DR, granzyme B, caspase-3 and CD56 can be useful for making the diagnosis of ACR.
Key Words: Renal transplantation; Graft rejection; Cellular immunity; Immunohistochemistry