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The Korean Journal of Pathology 2006;40(1): 46-51.
Effects of Genistein and Daidzein on the Growth of Human Colon Cancer HCT-116 Cells.
Jong Heon Shin, Ku Seong Kang, Joung Ok Kim, Ghil Suk Yoon, Tae Gyun Kwon, Jung Wan Kim, Yoon Kyung Sohn
1Department of Pathology, Kyungpook National University, School of Medicine, Daegu 70-422, Korea. yksohn@knu.ac.kr
2Department of Urology, Kyungpook National University, School of Medicine, Daegu 70-422, Korea.
3Department of Oral Microbiology, Kyungpook National University, College of Dentistry, Daegu 70-422, Korea.
BACKGROUND: Genistein and daidzein are two major soybean isoflavones. They have received increasing attention because of their possible roles for cancer prevention. However, their mechanisms of action and molecular targets on the human colon cancer cells are not fully understood. METHODS: Human colon cancer HCT-116 cells were treated with genistein and daidzein to investigate their effects on the cell growth and this was analyzed with MTT assay. TUNEL assay and Hoechst33342 stain were carried out to identify apotosis. RESULTS: Daidzein was able to inhibit cell proliferation and induce apoptosis of the HCT-116 cells, but genistein didn't affect the cell growth. The ER antagonist ICI182780 didn't attenuate the antiproliferative and proapoptotic effects of daidzein: this means the effect of daidzein on the HCT-116 cells may not be dependent on the ER pathway. The other soybean isoflavone, genistein, attenuated the effects of daidzein on the HCT-116 cells and its mechanism should be elucidated. CONCLUSIONS: These data suggest that daidzein may act as a preventive agent on human colon cancer, and its mechanism of action doesn't involve the ER-dependent pathway.
Key Words: Genistein; Daidzein; Colon cancer; HCT-116 cell
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