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The Korean Journal of Pathology 2004;38(3): 157-164.
Expression of Tumor Necrosis Factor-alpha, Interleukin-1beta and Inducible Nitric Oxide Synthase after Stereotaxic Injection of Lipopolysaccharide in Rat Hippocampus.
Hoon Kyu Oh, Ku Seong Kang, Ji Yeon Kim, Eun Kyoung Kwak, Jung Wan Kim, Ji Young Park, Yoon Kyung Sohn
1Department of Pathology, School of Medicine, Kyungpook National University, Daegu, Korea. yksohn@knu.ac.kr
2Department of Pathology, School of Medicine, Catholic University of Daegu, Korea.
3Department of Oral Pathology, School of Dentistry, Kyungpook National University, Daegu, Korea.
4Department of Oral Microbiology, School of Dentistry, Kyungpook National University, Daegu, Korea.
5Department of Pathology, Samsung Cheil Hospital, Sunkyunkwan University, School of Medicine, Seoul, Korea.
ABSTRACT
BACKGROUND: Brain inducible nitric oxide synthase (iNOS) might be detectable in several pathologic conditions, and it is thought to play an important role in their pathophysiology. Tumor necrosis factor (TNF)-alpha and interleukin (IL)-1beta are believed to be essential factors of iNOS induction of the brain. METHODS: After intrahippocampal stereotaxic injection of lipopoly-saccharide (LPS), the rat brains were removed at 6, 12 and 24 h. The rat brain tissues were examined to clarify the expression patterns of TNF-alpha, IL-1beta and iNOS. RESULTS: The inflammatory cells which were stained with anti-TNF-alpha antibody, appeared in 6 h and increased for 24 h after LPS injection. The iNOS positive cells appeared after 12 h of LPS injection. A semiquantitative analysis of reverse transcription-polymerase chain reaction (RT-PCR) revealed that the TNF-alpha and IL-1beta mRNA arose at 1 h, peaked at 6 h and then declined until 48 h after LPS injection. The iNOS mRNA arose after 6 h, peaked at 12 h, and declined until 48 h after LPS injection. CONCLUSIONS: We conclude that the induction of inflammatory events by intrahippocampal injection of LPS activates TNF-alpha and IL-1beta secretion, and this is followed by an induction of iNOS expression. TNF-alpha and IL-1beta seem to be related with iNOS expression in brain inflammation.
Key Words: Inducible Nitric Oxide Synthase; Tumor Necrosis Factor; nterleukins; Lipopoly; saccharides; Hippocampus
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