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The Korean Journal of Pathology 2001;35(2): 129-136.
A Study on the Expression of Proliferating Cell Nuclear Antigen and Apoptosis of the Hepatocellular Carcinoma in Human and Hepatitis B Virus X Transgenic Mice.
Hyung Bae Moon, Dae Yeul Yu, Hyung Ryun Yoo, Byung Joon So, Kwon Mook Chae, Haak Cheol Kim, Ki Jung Yun, Won Cheol Han, Hyang Jeong Jo, Bo Yong Kim
1Departments of Pathology, Surgery, and Internal Medicine, Wonkwang University, School of Medicine, Iksan 570-749, Korea. hbmoon@wonkwang.ac.kr
2Korea Research Institute of Bioscience and Biotechnology, Taejon, Korea.
ABSTRACT
BACKGROUND: This experiment was designed to study the cell kinetics of hepatocellular carcinoma (HCC) in both hepatitis B virus X (HBx) transgenic mice and humans. METHODS: The immunohistochemical stain of proliferating cell nuclear antigen (PCNA) and TdT-mediated dUTP-biotin nick end labeling (TUNEL) assay of apoptosis were used on formalin fixed-paraffin embedded tissues. RESULTS: PCNA labeling indices (PCNA-LI) in the liver of HBx transgenic mice were markedly increased in HCC (11.3%) compare to the dysplastic areas (1.3%) and in the liver of non-transgenic littermates (0.1%). There was no significant difference of PCNA-LI in the dysplastic areas between HCC developed mice and non-HCC developed mice. Apoptosis labeling indices (Apoptosis-LI) in both the dysplastic areas and HCC of HBx transgenic mice were similar to those of non-transgenic littermates. PCNA-LI was markedly increased in human HCC (28.9%) compare to the background of HCC (2.9%) and the control liver (2.9%). Apoptosis-LI was decreased in human HCC (0.3%) compare to the background of HCC (0.4%) and the control liver (1.0%). Conclusion : There is a marked increase of cell proliferating activity in human HCC and in HCC of HBx transgenic mice, and there is a decrease of apoptosis in human HCC, but not in HCC of HBx transgenic mice.
Key Words: Transgenic; Hepatocellular; Carcinoma; Proliferating cell nuclear antigen; Apoptosis