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Lee, Cho, Choi, Hwang, and Oh: Metastatic leiomyosarcoma of the thyroid gland: cytologic findings and differential diagnosis

Abstract

Metastatic leiomyosarcoma to the thyroid is an extremely rare occurrence, and only 18 cases have been reported. Here, we report a case of a 37-year-old woman who presented with multiple masses on the scalp. Excisional biopsy was done and the mass revealed fascicles of smooth muscle fibers which showed positive staining for smooth muscle actin, thus confirming the diagnosis of leiomyosarcoma. The patient was also found to have a 0.9 cm mass within the left thyroid. Fine-needle aspiration was done and the cytological smear showed hypercellular spindle cell clusters with hyperchromatic and large nuclei. Normal thyroid follicular cells were found within or around tumor cells. In this report, we present the cytologic findings of metastatic leiomyosarcoma to the thyroid and offer differential diagnoses of the aspirated spindle cells.

Soft tissue sarcoma metastatic to the thyroid is extremely rare, and it poses a challenge for a pathologist to diagnose it on cytology aspiration alone. Here, we report a case of metastatic leiomyosarcoma to the thyroid and describe in detail its cytologic findings.

CASE REPORT

A 37-year-old woman presented with multiple masses on the scalp. She underwent an excisional biopsy of the scalp mass at a local clinic and visited our hospital for further work up. Computed tomography of the neck revealed a poorly enhancing mass lesion in the left lobe of the thyroid measuring about 1 cm with ipsilateral cervical lymph node metastasis (Fig. 1A). An ultrasonographic examination of the thyroid revealed a 0.9 cm hypoechoic mass without increased vascularity (Fig. 1B). Ultrasound-guided fine-needle aspiration (FNA) was done to rule out the possibility of primary thyroid cancer. Unlike a typical thyroid malignancy, the aspirated material was sticky and difficult to aspirate.
The alcohol-fixed smears were stained with hematoxylin and eosin and Papanicolau for the cytologic examination. The cytological smears were hypercellular and displayed spindle cell clusters in hemorrhagic background. No necrotic material was observed. The spindle cells were arranged in a fascicular pattern and showed an indistinct cell border. The cells had eosinophilic and fibrillary cytoplasm. Individual cells showed variation in nuclear size and shape. However, the nuclei of most of the cells were hyperchromatic and large, and they demonstrated an elongated shape with blunt ends (Fig. 2A, B). Normal follicular cells were admixed with pleomorphic tumor cells, and were also observed around tumor cell clusters (Fig. 2C, D). These features raised the suspicion for a malignant tumor of mesenchymal origin.
Histological slides from the resection specimen of the scalp mass revealed a small nodular mass measuring 0.8 × 0.6 cm. The tumor cells were arranged in bundles or fascicles of smooth muscle fibers (Fig. 3A). They were highly pleomorphic and had a centrally located, blunted, cigar-shaped nucleus adjacent to perinuclear cytoplasmic vacuoles. The mitotic rate was high (7 mitosis per 10 high-power field), but there was no area of necrosis. Immunohistochemical staining showed a positive result for smooth muscle actin (SMA) and desmin (Fig. 3).
Positron emission tomography–computed tomography revealed several hypermetabolic masses on the right side of the abdomen, the largest of which measured 5.8 cm. One mass was located in the mesentery, which had no association with the uterus. In addition, there were multiple hypermetabolic lesions in the left internal mammary area, both thigh, and the lung. To exclude other spindle cell tumors which can arise in the abdominal cavity, immunostaining for S100, CD34, MDM2, c-Kit, and anaplastic lymphoma kinase were performed. All of the additional immunohistochemical stainings turned out negative, and the possibility of schwannoma, solitary fibrous tumor, liposarcoma, gastrointestinal stromal tumor and inflammatory myofibroblastic tumor were ruled out, respectively. Considering all the clinical, morphological and immunohistochemical features, we determined that the final diagnosis of the tumor located in the thyroid gland to be metastatic leiomyosarcoma from the mesentery. Because of the presence of metastatic disease, the patient received doxorubicin-based chemotherapy instead of further surgical intervention. She is still alive 22 months after her first visit.

DISCUSSION

Metastasis of sarcoma to the thyroid is rare with a few cases reported in the literature. To date, 18 cases have been reported as metastatic leiomyosarcoma to the thyroid, most of which are from uterine leiomyosarcoma (Table 1). Furthermore, it is very rare to be diagnosed using FNA cytology [13].
FNA cytology of metastatic leiomyosarcoma tends to show hypercellular spindle cell clusters with marked pleomorphism, and there can be conspicuous mitoses. Therefore, the differential diagnosis should include primary thyroid tumors showing spindle cell features; such as medullary carcinoma, anaplastic carcinoma and spindle epithelial tumor with thymus-like differentiation (SETTLE). Likewise, primary or metastatic mesenchymal tumors like malignant peripheral nerve sheath tumor (MPNST) or synovial sarcoma should be considered.
Medullary thyroid carcinoma is one of the first differential diagnosis to consider when spindle cells are observed in thyroid FNA. However, the characteristic cytology of medullary carcinoma is dispersed single cells having “salt-and-pepper” nuclei. In most cases, oval-to-plasmacytoid cells were observed together.
Cytomorphologically, SETTLE has a close resemblance to leiomyosarcoma. They can be shown as variably sized groups of spindle cells with uniform, elongated, and cigar-shaped nuclei similar to those of leiomyosarcoma [4]. In addition, epithelial components of SETTLE can mimic benign follicular cells that appear in metastatic leiomyosarcoma. However, diffuse immunoreactivity for cytokeratin in both components is the most obvious difference from leiomyosarcoma.
Anaplastic carcinomas also have spindle-shaped cells. However, anaplastic carcinomas rarely occur below the age of 50 and usually present as a rapidly growing mass infiltrating surrounding soft tissues. Spindle cells in anaplastic carcinoma show a more bizarre morphology compared to that shown in leiomyosarcoma [19]. Osteoclast-like giant cells, necrosis, and mitotic figures are a common feature, and in more than half of the case, anaplastic carcinomas are positive for cytokeratin. PAX8 is positive in about 80% of anaplastic carcinomas.
MPNST can present as highly cellular clusters of spindle cells showing wavy, elongated nuclei with tapered ends [20]. The cytologic features of synovial sarcoma include hypercellular and uniform oval-to-spindle cells without nuclear pleomorphism or necrosis. Despite its biphasic nature, the epithelial component is rarely observed in the FNA specimen.
Although there are differences between the differential diagnoses as described above, it is nearly impossible to conclusively diagnose leiomyosarcoma in FNA slides with only histologic observation of the cells in cytologic smears. Immunohistochemistry is crucial for a precise diagnosis. Leiomyosarcoma is positive for vimentin, SMA, muscle-specific actin, desmin and h-caldesmon and negative for cytokeratin, thyroglobulin, calcitonin, and S-100 protein. In our case, we diagnosed leiomyosarcoma by confirming that the resected scalp mass was positive for SMA and desmin and negative for S-100 protein, CD34, MDM2, c-Kit, and anaplastic lymphoma kinase. It is difficult to make a distinction between primary and metastatic leiomyosarcoma cytologically. Metastases to the thyroid gland occur more frequently than is generally appreciated and can be easily misdiagnosed as a primary thyroid tumor. Given the rarity of thyroid primary sarcoma, pathologists should be alert to the possibility of metastatic tumor, especially when benign follicular cells are identified within or around malignant cells.

Notes

Ethics Statement

This study was approved by the Institutional Review Board of Samsung Medical Center with a waiver of informed consent (IRB No. 2021-03-125).

Availability of Data and Material

The datasets generated or analyzed during the study are available from the corresponding author on reasonable request.

Code Availability

Not applicable.

Author Contributions

Conceptualization: YLO. Data curation: JL. Formal analysis: JL. Investigation: JL, YC, IH, KHC, YLO. Methodology: JL, YC. Resources: YLO. Supervision: YLO. Validation: YLO, JL. Visualization: JL, YC. Writing—original draft: JL. Writing—review & editing: YLO, YC. Approval of final manuscript: all authors.

Conflicts of Interest

The authors declare that they have no potential conflicts of interest.

Funding Statement

No funding to declare.

Fig. 1
Radiologic findings of metastatic leiomyosarcoma. (A) Computed tomography scan reveals 1 cm sized poorly enhancing space occupying lesion (arrow) in left thyroid. (B) Ultrasound shows marked hypoechoic nodule in the inferior pole of left thyroid.
jptm-2021-06-23f1.jpg
Fig. 2
Cytologic features of metastatic leiomyosarcoma. (A) Hypercellular spindle cell clusters are arranged in a fascicular pattern. (B) The cells show indistinct cell borders, eosinophilic cytoplasm, and hyperchromatic, large, elongated nuclei with blunt ends. (C, D) Normal follicular cells are admixed with pleomorphic tumor cells (C) or discovered around tumor cell clusters (D).
jptm-2021-06-23f2.jpg
Fig. 3
Histologic features of the scalp mass. (A) Excisional biopsy specimen from the scalp mass shows bundles or fascicles of smooth muscle fibers. The tumor cells are highly pleomorphic and have a centrally located, blunted, cigar-shaped nucleus adjacent to perinuclear cytoplasmic vacuoles. (B, C) Smooth muscle actin (B) and desmin (C) immunostaining shows positivity. (D) c-Kit immunostaining shows negativity.
jptm-2021-06-23f3.jpg
Table 1
Clinicopathlogical and immunohistochemical data on 18 reported cases of metastatic leiomyosarcoma of the thyroid gland
Author Age (yr) Sex Origin Chief complaint Location/size Cytology Pathology Vimentin Desmin SMA CD34 S100 Cytokeratin Thyroglobulin
Gross and Horton (1975) [5] 46 M Great saphenous vein Painless swelling in neck N/M - - - - - - - - -
Cruickshank (1988) (as cited in Vankalakunti et al. [6]) 30 F Uterus Diffuse thyroid swelling N/M - - - - - - - - -
Gattuso et al. (1989) [2] N/M N/M Retroperitoneal N/M Left thyroid/3 cm - - - - - - - - -
Bode-Lesniewska et al. (1994) [7] 69 F Duodenum N/M 5 cm - - - - - - - - -
Michelow (1995) (as cited in Vankalakunt et al. [6]) N/M N/M Uterus Thyroid nodular swelling N/M - - - - - - - - -
Nakhjavani (1997) (as cited in Vankalakunti et al. [6]) N/M N/M Uterus N/M N/M - - - - - - - - -
Wang et al. (1998) [8] 63 F Left leg Rapidly progressive enlargement of the neck N/M - - - - - - - - -
Chen et al. 1999) [9] N/M N/M Stomach N/M Unilateral Anaplastic, malignant spindle cell tumor N/D - - - - - - -
Leath et al. (2002) [10] 5th decade F Uterus Postmenopausal bleeding Diffuse and numerous/up to 2.9 cm N/D Smooth muscle cells with atypia and mitotic figures N/D Strong positive Focal positive N/D N/D N/D N/D
D’Andrea et al. (2003) [11] 60 F Pulmonary artery Dyspnea and hemoptysis Both thyroid/up to 4.7 cm N/D N/D - - - - - - -
Deng et al. (2005) [12] N/M N/M Leg N/M N/M - - - - - - - - -
Giannikaki et al. (2006) [13] 54 F Uterus Single “cold” nodule in the right thyroid Right thyroid/0.12 cm co-existing with PTC N/D Spindle cells with prominent nuclear atypia and mitoses N/D Positive Positive N/D N/D Negative N/D
Eloy et al. (2007) [14] 54 F Uterus Rapidly enlarging and painful left neck mass Left thyroid/2.5 cm Consistent with a sarcomatous lesion Large cells with hypercellularity, nuclear atypia, and pleomorphism with atypical mitoses Positive Negative Negative N/D Negative Negative Negative
Nemenqani et al. (2010) [1] 86 F Pelvic soft tissue Right thyroid swelling Right thyroid Hypercellular spindle cell proliferation arranged in sheets mixed with stroma; marked pleomorphism; large hyperchromatic nuclei; numerous mitoses Numerous mitoses and bizarre nuclei Positive Negative Positive Negative Negative Negative Negative
Young et al. (2011) [15] 55 F Lung Cough N/M N/D Fascicular pattern of spindle-shaped cells with increased cellularity and hyperchromatic, bizarre, malformed, and megakaryocytic nuclei Positive Focal positive Positive Negative Negative N/D N/D
Gauthe et al. (2017) [16] 47 F Uterus Fast growing thyroid nodule N/M N/D N/D N/D N/D N/D N/D N/D N/D N/D
Golovko et al. (2020) [17] 65 F Vagina Multinodular toxic goiter Right thyroid Suggestive of the benign thyroid lesion Consistent with metastatic vaginal leiomyosarcoma N/D N/D N/D N/D N/D N/D N/D
Irizarry-Villafane et al. (2020) [18] 47 F Uterus Neck discomfort Left upper lobe/2.4 cm Marked cellularity of atypical spindle cells, dyscohesive, and in tissue aggregates with some binucleated cells Spindle cell tumor with frequent mitotic figures N/D Positive Positive N/D N/D Negative Negative

SMA, smooth muscle actin; M, male; F, female; N/M, not mentioned; N/D, not done.

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