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JPTM > Volume 52(2); 2018 > Article
Wu and Hsieh: Denosumab-Treated Giant Cell Tumor of the Bone Mimicking Low-Grade Central Osteosarcoma
Giant cell tumor of the bone is a benign but locally aggressive bone tumor. In recent years, denosumab, a receptor activator for nuclear factor-κβ ligand (RANKL) inhibitor has emerged as a treatment alternative, especially when surgical clearance cannot be obtained or for frequent recurrence. However, treatment-related histologic changes have been rarely documented in the literature until recently and show substantial overlap with other primary tumors of bone such as low-grade central osteosarcoma, which can easily lead to a misdiagnosis. Here we present a typical case of denosumab-treated giant cell tumor of the bone confirmed by detection of an H3F3A (histone H3 family 3A protein) mutation, which we believe is the first case report in Taiwan.

CASE REPORT

A 50-year-old female patient visited our clinic complaining of left knee pain that had been present for 4 months. The condition was aggravated during ambulation and worsened as time went by. There was no trauma history or any significant past medical history of note. She had gone to another hospital first where a left distal femur osteolytic tumor was found. Needle biopsy was performed there, and the pathology revealed giant cell tumor of the bone (Fig. 1A). She was then given subcutaneous injections of denosumab (120 mg) every 4 weeks for 3 months. Due to personal reasons, she visited our clinic thereafter. Magnetic resonance imaging showed a 4.1-cm expansile, eccentric osteolytic bone tumor around the epiphysis and metaphysis of the left distal femur; the lesion manifested low T1 weighted image and T2 weighted image with strong contrast enhancement (Fig. 1B). Relatively well-defined margins with cortical thinning were noted. No conspicuous tumor regression was noted, and the lesion was still radiologically classified as grade II using the Campanacci classification. After 3 months of denosumab treatment, adequate curettage and adjuvant cryotherapy with liquid nitrogen and bone graft implantation were performed.
A brownish circumscribed tumor, 5 cm in size, was found during the operation, with no soft tissue or joint involvement. The curettage specimen was sent for pathologic examination. Light microscopy showed that the tumor was composed mostly of woven bone, with a small amount of aggregated mononuclear cell foci and scattered osteoclast-like giant cells (< 10%). Compared with ordinary giant cell tumors of the bone before denosumab treatment (Fig. 1A), this post-treatment specimen showed decreased cellularity, reduced number and size of osteoclast-like giant cells, and abundant new bone deposition as broad, rounded cords, or long, curvilinear arrays (Fig. 1C, D). Only minimal cytological atypia and occasional mitoses were noted. Focal necrosis was identified. Immunohistochemically, the tumor cells were negative for cyclin-dependent kinase 4 (1:200, ZETA Corporation, Arcadia, CA, USA), and murine double minute 2 (MDM2; 1:50, ZETA Corporation) (Fig. 1E). The genetic study was negative for MDM2 amplification. In addition, the H3F3A gene mutation was assessed with polymerase chain reaction and direct sequencing which showed hotspot mutation c.100G > T (G34W) (Fig. 1F).
After the operation, the range of motion (ROM) for the left knee gradually improved from partial to full ROM at 6-month follow-up. No evidence of tumor recurrence was noted.
This study was approved by the Institutional Review Board of Kaohsiung Veterans General Hospital (IRB No. VGHKS18-CT2-01) with a waiver of informed consent.

DISCUSSION

Giant cell tumor of the bone is composed of a proliferation of mononuclear cells amongst which are scattered numerous macrophages and large osteoclast-like giant cells. It is thought that the numerous large osteoclast-like giant cells are not neoplastic but reactive in nature [1]. These neoplastic mononuclear cells exhibit mutations to the H3F3A gene [2], and express high levels of RANKL [3], which binds to RANK, a receptor expressed on the surface of multinucleated osteoclast-like giant cells and their precursors. This results in the activation and proliferation of these cells, leading to bone resorption [4]. Denosumab, a RANKL inhibitor, has been shown to retard or arrest tumor growth [5]. Denosumabtreated tumors can be devoid of giant and mononuclear cells and are composed of abundant woven bone and fibrous tissue.
Wojcik et al. [6] reported nine cases and demonstrated that all treated giant cell tumors of the bone showed marked giant cell depletion. A gradual decrease in stromal cellularity and increased new bone formation appeared to correlate with treatment. Denosumab shifts the balance of physiological bone formation away from RANK-mediated osteoclastic bone resorption toward osteoprotegrin-induced bone formation. However, it seems that this shift does not reflect terminal differentiation, as the morphology reverts to classic giant cell tumor histopathology upon denosumab therapy cessation.
The combination of relatively bland-looking tumor cells with minimal cytological atypia, as well as abundant new bone formation in curvilinear arrays within a fibrous background, produced a resemblance to low-grade central osteosarcoma [6]. In addition, benign multinucleated giant cells have been reported in up to 36% of low-grade central osteosarcomas [7], making the distinction more challenging. Recently, Rekhi et al. [8] also reported that after denosumab treatment, giant cell tumors of the bone can appear as low-grade osteosarcomas on histopathologic examination, but lack the clinical behavior of an osteosarcoma.
In terms of differential diagnoses, giant cell-rich conventional osteosarcoma and malignancy in giant cell tumor of the bone are characterized by more severe cytological atypia and more prominent mitotic activity, which were not seen in this case. Low-grade central osteosarcoma was also excluded due to the lack of an infiltrative growth pattern and negative MDM2 amplification. Finally, we performed a genetic test and an H3F3A hotspot mutation (G34W) was detected. This finding supported the diagnosis of giant cell tumor of the bone.
In summary, we present a case of giant cell tumor of the bone with typical morphologic changes after denosumab treatment, including giant cell depletion and abundant new bone formation. Detection of an H3F3A hotspot mutation (G34W) also confirmed the diagnosis. This is the first case report in Taiwan. Because denosumab-treated tumors bear little resemblance to their pretreatment counterparts and have substantial histologic overlap with other primary tumors of the bone, it is important for pathologists to understand the differences between treatment-related histologic changes and morphologic mimics. Due to the increasing use of denosumab in recent years, it is crucial to pay careful attention to the history of denosumab administration to avoid a misdiagnosis.

Notes

Conflicts of Interest

No potential conflict of interest relevant to this article was reported.

Acknowledgments

We thank Dr. Hsuan-Ying Huang, the director of Department of Pathology, Kaohsiung Chang Gung Memorial Hospital, who helped us perform the molecular study of MDM2 amplification and H3F3A mutation.

Fig. 1.
(A) The typical picture of giant cell tumor before treatment shows “giant” osteoclasts with >20 and sometimes >50 nuclei. (B) Magnetic resonance imaging reveals an expansile, eccentric osteolytic bone tumor around epiphysis and metaphysis of left distal femur. (C, D) Compared with ordinary giant cell tumor, this case exhibits reduced number and size of osteoclast-like giant cells and also abundant new bone deposition, mimicking low-grade central osteosarcoma. (E) The immunohistochemical result shows negative for MDM2. (F) H3F3A hotspot mutation (G34W) is detected.
jptm-2016-12-21f1.tif

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