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The Korean Journal of Pathology 1984;18(3): 249-256.
CA 전기영동법을 이용한 HDL-C 치 및 total cholesterol/HDL-C, LDL-C/HDL-C 치 측정
HDL-C, Total Cholesterol/HDL-C, and LDL-C/HDL C determination by Cellulose Acetate Electrophoresis
ABSTRACT
Previously we mainly used the precipitation method for measuring HDL cholesterol which was closely related to coronary heart disease. Now the author performed HDL-cholesterol determination against normal healthy adults, diabetics, patients of heart disease with or without myocardical infarction, and hypertension by CA electrophoresis and total cholesterol with V-cholestase(Nissui). Afterthat, we measured the ratio of total cholesterol/HDL-C and LDL-C/HDL-C in each group, and then we compared with normal control. The electrophoretic pattern of HDL cholesterol was corresponding to alpha-lipoprotein. The HDL cholesterol levels of normal male and female were 54.6±11.0㎎/dl and 71.7±13.0㎎/dl. The levels in myocardial infarction were 40.7±13.4㎎/dl in male and 39.7±13.1㎎/dl in female. Those of heart disease without myocardial infarction were 43.1±21.1㎎/dl in male and 51.0±21.8㎎/dl in female. The average HDL-C levels of these disease groups were lower than the normal control about 10㎎/dl this data was statistically significant. In hypertensive disease group, the levels were 45.2±16.5㎎/dl in male, 61.6±9.7㎎/dl in female and those of diabetics were 53.7±25.3㎎/dl in male, 55.8±11.8㎎/dl in female. There was little sexual difference in diabetics. In the ratio of total cholesterol/HDL-C and LDL-C/HDL-C, normal male was 3.62±0.66, 2.37±0.61 and normal female 2.61±0.40, 1.37±0.32. Of the mycardial infarction, male 5.31±2.96, 3.81±2.84, female 5.55±1.47, 3.94±1.33. There was statistically important difference between normal and myocardial infarction. Of the other cardiovaccular disease, male was 5.13±1.52, 3.63±1.83 and in female diabetics, total cholesterol/HDL-C was 5.07±2.64. Of the se cases, a little importance in comparison with normal. The remainings had a no statistically importance.
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