Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81

Warning: fopen(upload/ip_log/ip_log_2024-03.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83

Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84
Studies on Anaerobe Infection: Use of Gas Liquid Chromatography for Anaerobe Identification
Skip Navigation
Skip to contents

J Pathol Transl Med : Journal of Pathology and Translational Medicine

OPEN ACCESS
SEARCH
Search

Articles

Page Path
HOME > J Pathol Transl Med > Volume 15(2); 1981 > Article
Etc Studies on Anaerobe Infection: Use of Gas Liquid Chromatography for Anaerobe Identification
Journal of Pathology and Translational Medicine 1981;15(2):110-118
DOI: https://doi.org/
Department of Clinical Pathology, Yonsei University College of Medicine
  • 1,076 Views
  • 10 Download
  • 0 Crossref
  • 0 Scopus

Anaerobic bacteria are organisms that could grow only in the abscence of free oxygen. Therefore it is very difficult to culture the organisms. Anaerobic infections are often neglected not only because of the difficulty of cultivation but also of identification which require analysis of fattyacids. As the isolates in Korea have not been subjected to fatty acid analysis, our knowledge in anaerobe infections are very limited. This study was conducted to identify clinical isolates of anaerobes accurately with the use of gas liquid chromatography(GLC) and to determine the relationship between anaerobe species and the infection. Clinical specimens obtained at YUMC during the period of September 1979-August 1980 were cultured anaerobically and the anaerobes isolated were identified with GLC if necessary. Clinical conditions were reviewed retrospectively. Following results were obtained. 1) A total of 342 anaerobic organisms were isolated from 282 clinical specimens. Most frequently isolated anaerobes were Pc. asaccharolyticus, Ps. anaerobius, S. intermedius, Cl. perfringens, B. fragilis and B. melaninogenicus. 2) Anaerobes were most frequently isolated from specimens of thorax, abdomen and soft tissue wound. 3) With the review of 118 available patients' records, it was found that most frequent clinical conditions were appendicitis with peritonitis, wound infection following GIT cancer surgery, empyema, lung abscess, and infection of umblicus of new-torn and soft tissues. 4) Only 18.4% of the specimens yielded anaerobes alone, and the remaining 81.6% yielded both anaerobes mixed with aerobes. 5) With the use of GLC, up to 94.4% and 94.1% of isolates unidentifiable by conventional methods, were satisfactorily identified during the periods of September 1979-May 1980 and June 1980-August 1980 respectively.

Related articles

J Pathol Transl Med : Journal of Pathology and Translational Medicine