Abstract

The results of NBT test were found very depending on how the testis performed. NBT tests performed simultaneously on various concentration of heparin, various length of incubation time, different mixing instruments, venous blood and capillary blood, and scoring of three good trained technicians, from 90 healthy individuals. Heparin increased proportions of NBT positive neutrophils and lowest possible concentration should be used. NBT tests showed a considerably higher proportion of NBT positive neutrophils in capillary blood than in venous blood. 15 minutes incubation time at 37℃ affected unstable and obscure formazan deposits on NBT positive neutrophils than 25 minutes. Scoring of different good trained technicians are the same results on NBT positive neutrophils.