| Home | E-Submission | Sitemap | Contact Us |  
The Korean Journal of Pathology 1975;9(1): 55-60.
순수 임파구 분리에 관한 연구
Separation of Lymphocytes from Human Peripheral Blood
ABSTRACT
This paper describes a technique for the isolation of lymphocytes from haparinized human peripheral blood. When whole blood was layered on top of a mixture of Ficoll and Isopaque and gradient centrifuged at room temperature, the cellular elements of whole blood were divided into two main parts, a lymphocyte bone in plasma layer and a layer consisting of sedimented granulocytes and erythrocytes at the bottom of the tube. By this method the isolation of viable lymphocyte with 96.6% purity was possible in a short period of time. Impurities found in the lymphocyte zone was granulocytes; 0.2%, monocytes; 2.8%, eosinophils; 0.4%, erythrocytes; less than 5% of total cell counts respectively. Isolating conditions and purities together with detailed directions are presented. It is shown in the present paper that adopting the procedure introduced a lymphocyte suspension of extreme purity with high viability may be obtained for lymphocyte research.
TOOLS
PDF Links  PDF Links
Full text via DOI  Full text via DOI
Download Citation  Download Citation
CrossRef TDM  CrossRef TDM
  E-Mail
Share:      
METRICS
710
View
0
Download
Related article
Studies on Blastogenesis and T-Rostte Formation of Human Peripheral Blood Lymphocyte  1977 July;11(2)