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The Korean Journal of Pathology 1968;2(2): 143-147.
각종분광광도계에 있어서 cyanmethemoglobin의 흡광 계수
1, 1, 1, 2
1우석대학교 의과대학 임상병리과
2우석대학교병설 의학기술초급대학 임상병리과
Extinction Coefficient of Cyanmethemoglobin in various Spectrophotometers
Ki Hong Kim1, IK Whan Lee1, Yong Myung Kim1, Joo Sup Lee2
1Department of Clinical Pathology, Woo-Sok University, Medical College,Seoul, Korea
2Department of Clinical Pathology, Woo Sok University, Junior College of Medical, Seoul, Korea
ABSTRACT
Drabkin's cyanmethemoglobin method is acknowledged as the best available photometric procedure for the determination of total hemoglobin in blood. In Korea standard hemoglobin (Acuglobin, Ortho Co.) has been not imported, so cyanmethemoglobin method in hemoglobinometry is not widely used in many hospitals which are equiped with spectrophotometer. Only a few hospitals are available for this method by using Fisher Hemophotometer. Hemoglobin, however, can be calculated without standard solution by knowing extinction coefficient of cyanmethemoglobin in various photometers. Authors present the extinction coefficient in Beckman DU, Beckman B, Coleman Jr. 6, and Spectronic 20.
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