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The Korean Journal of Pathology 1995;29(4): 415-422.
Application of Gene Rearrangement Analysis for Diagnosis of Malignant Lymphoma.
Kyung Soo Kim, Chan Choi
1Department of Pathology, Chonnam University Medical School, Kwangju, Korea.
2Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Science, North Carolina, USA.
To evaluate the utility of gene rearrangement analysis, eight cases of malignant lymphoma, one case of Hodgkin's disease, two cases of angioiminunoblastic lymphadenopathy (AILD) and two cases of non-specific lymphadenitis were studied by immunohistochemical and genetic analysis. Southern blot analysis was perfon-ned by a using vacuum transfer system and a biotin labelled probe. This method was faster, safer, and more convenient than conventional methods. Gene rearrangement study showed rearranged novel bands in five of six cases of B cell lymphoma, in all cases of T cell lymphoma, and in all cases of AILD. No rearrangement of the B cell receptor(BCR) or of the T cell receptor(TCR) was seen in Hodgkin's disease or in nonspecific lymphadenitis. These results suggest that gene rearrangement analysis of BCR and TCR is a recommended method for the diagnosis of clonality in lymphoproliferative disorders. It would allow pathologists to differentiate lymphoma from polyclonal lymphoid proliferation and to provide information for cell lineage.
Key Words: Gene rearrangement analysis; Malignant lymphoma; B cell receptor; T cell receptor