Warning: mkdir(): Permission denied in /home/virtual/lib/view_data.php on line 81

Warning: fopen(upload/ip_log/ip_log_2024-03.txt): failed to open stream: No such file or directory in /home/virtual/lib/view_data.php on line 83

Warning: fwrite() expects parameter 1 to be resource, boolean given in /home/virtual/lib/view_data.php on line 84
Application of Gene Rearrangement Analysis for Diagnosis of Malignant Lymphoma.
Skip Navigation
Skip to contents

J Pathol Transl Med : Journal of Pathology and Translational Medicine

OPEN ACCESS
SEARCH
Search

Articles

Page Path
HOME > J Pathol Transl Med > Volume 29(4); 1995 > Article
Original Article Application of Gene Rearrangement Analysis for Diagnosis of Malignant Lymphoma.
Kyung Soo Kim, Chan Choi
Journal of Pathology and Translational Medicine 1995;29(4):415-422
DOI: https://doi.org/
1Department of Pathology, Chonnam University Medical School, Kwangju, Korea.
2Laboratory of Molecular Carcinogenesis, National Institute of Environmental Health Science, North Carolina, USA.
  • 1,556 Views
  • 13 Download
  • 0 Crossref
  • 0 Scopus

To evaluate the utility of gene rearrangement analysis, eight cases of malignant lymphoma, one case of Hodgkin's disease, two cases of angioiminunoblastic lymphadenopathy (AILD) and two cases of non-specific lymphadenitis were studied by immunohistochemical and genetic analysis. Southern blot analysis was perfon-ned by a using vacuum transfer system and a biotin labelled probe. This method was faster, safer, and more convenient than conventional methods. Gene rearrangement study showed rearranged novel bands in five of six cases of B cell lymphoma, in all cases of T cell lymphoma, and in all cases of AILD. No rearrangement of the B cell receptor(BCR) or of the T cell receptor(TCR) was seen in Hodgkin's disease or in nonspecific lymphadenitis. These results suggest that gene rearrangement analysis of BCR and TCR is a recommended method for the diagnosis of clonality in lymphoproliferative disorders. It would allow pathologists to differentiate lymphoma from polyclonal lymphoid proliferation and to provide information for cell lineage.

Related articles

J Pathol Transl Med : Journal of Pathology and Translational Medicine