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The Korean Journal of Pathology 1994;28(4): 376-380.
Overexpression of Mutant p53 in Human Anaplastic Astrocytoma and Glioblastoma Multiforme.
Sang Sook Lee, Kam Rae Cho, Cheoul Hee Yun, Sang Pyo Kim, Kwan Kyu Park, Eun Sook Chang, Eun Ik Sohn
1Department of Pathology, Keimyung University School of Medicine, Taegu, Korea.
2Department of Neurosurgery, Keimyung University School of Medicine, Taegu, Korea.
ABSTRACT
A total of 30 cases of cerebral gliomas, including 6 cases of low grade astrocytomas, 6 anaplasticastrocytoomas and l8 glioblastomas multiforme, was examined immunohistochemically to demonstrate the overexpression of mutant forms of p53 protein and to evaluate their relationships with histological subtypes. A p53 monoclonal antibody was applied to the routine formalin fixed, paraffin-embedded tissues for this study using microwave-assisted avidin-biotin method. Overexpression of p53 protein was identified in 4 out of 6 anaplastic astrocytomas (66.7%) and in l3 out of l8 glioblastomas multiforme (72.2%). No immunohistochemical positivity of p53 was found in adjacent normal brain tissue, gliosis and 6 cases of astrocytoma. These results suggest that overexpression of mutant p53 may be an important step in the development and progression of malignant astrocytoma, especially of the aggressive subtypes of glioma, including glioblastoma multiforme.
Key Words: p53; Anaplastic astrocytoma; Glioblastoma multiforme; Immunohistochemistry