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JPTM > Volume 52(5); 2018 > Article
Journal of Pathology and Translational Medicine 2018;52(5): 275-282.
doi: https://doi.org/10.4132/jptm.2018.07.29
Differential MicroRNA Expression between EGFR T790M and L858R Mutated Lung Cancer
Ji Yeon Kim1 , Woo Jeong Lee2, Ha Young Park3, Ahrong Kim4, Dong Hoon Shin5, Chang Hun Lee4
1Department of Pathology, Inje University Haeundae Paik Hospital, Busan, Korea
2Pusan National University School of Medicine, Yangsan, Korea
3Department of Pathology, Inje University Busan Paik Hospital, Busan, Korea
4Department of Pathology, Pusan National University Hospital, Busan, Korea
5Department of Pathology, Pusan National University Yangsan Hospital, Yangsan, Korea
Corresponding Author: Chang Hun Lee ,Tel: +82-51-240-7718, Fax: +82-51-242-7422, Email: cnlee@pusan.ac.kr
Received: May 16, 2018;  Revised: July 22, 2018  Accepted: July 29, 2018.  Published online: August 16, 2018.
ABSTRACT

Background:
MicroRNAs (miRNAs) are short, non-coding RNAs that mediate post-transcriptional gene regulation. They are commonly deregulated in human malignancies, including non-small cell lung cancer (NSCLC). The aim of this study is to investigate miRNA expression in T790M-mutated NSCLC resistant to epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors.
Methods:
Six cases of resected NSCLC harboring the T790M mutation were examined. We performed miRNA time polymerase chain reaction (PCR) array profiling using EGFR T790M-mutated NSCLC and L858R-mutated NSCLC. Once identified, miRNAs that were differentially expressed between the two groups were validated by quantitative real-time polymerase chain reaction (qRT-PCR).
Results:
miRNA PCR array profiling revealed three up-regulated miRNAs whose expression levels were altered 4.0-fold or more in the EGFR T790M mutation group than in the L858R group: miR-1 (fold change, 4.384), miR-196a (fold change, 4.138), and miR-124 (fold change, 4.132). The three differentially expressed miRNAs were validated by qRT-PCR, and they were found to be overexpressed in the T790M group relative to L858R group. In particular, expression levels of miR-1 and miR-124 were significantly higher in the T790M group (p-value of miR-1 = .004, miR-124 = .007, miR-196a = .096).
Conclusions:
MiR-1, miR-124, and miR-196a are overexpressed in EGFR T790M mutated NSCLC.
Key Words: MicroRNAs; Lung neoplasms; EGFR T790M mutation
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