Fig. 1Immunohistochemical expression of P2X7 receptor (P2X7R) in papillary thyroid carcinomas (PTCs). (A) Low expression of P2X7R; PTC with Hashimoto's thyroiditis (HT). (B) High expression of P2X7R; PTC without HT.
Table 1.Clinicopathological features of PTC according to presence of HT
Characteristic |
PTC with HT (n = 84) |
PTC without HT (n = 86) |
p-value |
Age at diagnosis (yr) |
47.1 ± 11.6 |
48.8 ± 12.2 |
.33 |
< 45 |
30 (35.7) |
22 (25.6) |
|
≥ 45 |
54 (64.3) |
64 (74.4) |
|
Gender |
|
|
.01 |
Male |
4 (4.7) |
14 (16.3) |
|
Female |
80 (95.3) |
72 (83.7) |
|
Histological variant |
|
|
.14 |
Variants |
13 (15.4) |
7 (8.1) |
|
Conventional |
71 (84.6) |
79 (91.9) |
|
Tumor multifocality |
|
|
.84 |
Yes |
31 (36.9) |
33 (38.4) |
|
No |
53 (63.1) |
53 (61.6) |
|
Tumor size (cm) |
|
|
.11 |
< 2 |
76 (90.4) |
70 (81.4) |
|
2-4 |
6 (7.1) |
15 (17.4) |
|
> 4 |
2 (2.5) |
1 (1.2) |
|
Lymphovascular invasion |
|
|
< .001 |
Yes |
24 (28.6) |
59 (68.6) |
|
No |
60 (71.4) |
27 (31.4) |
|
Extrathyroid extension |
|
|
< .001 |
Yes |
23 (27.4) |
55 (64) |
|
No |
61 (72.6) |
31 (36) |
|
Lymph node metastasis |
|
|
< .001 |
Yes |
20 (23.8) |
51 (59.3) |
|
No |
64 (76.2) |
35 (40.7) |
|
Table 2.P2X7R expression in papillary thyroid cancer
Characteristic |
P2X7R low expression (n = 80) |
P2X7R high expression (n = 90) |
p-value |
Age at diagnosis (yr) |
47.5 ± 12.2 |
48.4 ± 11.7 |
.13 |
< 45 |
29 (36.2) |
23 (25.6) |
|
≥ 45 |
51 (63.8) |
67 (74.4) |
|
Gender |
|
|
.08 |
Male |
5 (6.2) |
13 (14.4) |
|
Female |
75 (93.8) |
77 (85.6) |
|
Histological variant |
|
|
.78 |
Variants |
10 (12.5) |
10 (11.1) |
|
Conventional |
70 (87.5) |
80 (88.9) |
|
Tumor multifocality |
|
|
.12 |
Yes |
35 (43.8) |
29 (32.2) |
|
No |
45 (56.2) |
61 (67.8) |
|
Tumor size (cm) |
|
|
.55 |
< 2 |
70 (87.5) |
76 (84.4) |
|
2-4 |
8 (10) |
13 (14.4) |
|
> 4 |
2 (2.5) |
1 (1.2) |
|
Lymphovascular invasion |
|
|
< .001 |
Yes |
23 (28.8) |
60 (66.7) |
|
No |
57 (71.2) |
30 (33.3) |
|
Extrathyroid extension |
|
|
< .001 |
Yes |
20 (25) |
58 (64.4) |
|
No |
60 (75) |
32 (35.6) |
|
Lymph node metastasis |
|
|
< .001 |
Yes |
21 (26.2) |
50 (55.6) |
|
No |
59 (73.8) |
40 (44.4) |
|
Hashimoto’s thyroiditis |
|
|
< .001 |
Yes |
60 (75) |
24 (26.7) |
|
No |
20 (25) |
66 (73.3) |
|
Table 3.Multivariate analysis of factors potentially affecting the expression of P2X7R
Factor |
Odds ratio |
95% Confidence interval |
p-value |
Age |
1.42 |
0.63-3.21 |
.4 |
Gender |
0.75 |
0.2-2.8 |
.67 |
Multifocality |
1.15 |
0.31-3.99 |
.87 |
Lymphovascular invasion |
1.31 |
0.48-3.61 |
.59 |
Lymph node metastasis |
1.81 |
0.71-4.6 |
.21 |
Extrathyroid extension |
3.16 |
1.31-7.6 |
.01 |
Hashimoto’s thyroiditis |
5.43 |
2.45-12 |
< .001 |
Table 4.P2X7R expression in papillary thyroid cancer with Hashimoto's thyroiditis
Characteristic |
P2X7R (scores 0-2) (n = 60) |
P2X7R (scores 3-9) (n = 24) |
p-value |
Age at diagnosis (yr) |
|
|
.07 |
< 45 |
25 |
5 |
|
≥ 45 |
35 |
19 |
|
Gender |
|
|
.33 |
Male |
2 |
2 |
|
Female |
58 |
22 |
|
Histological variant |
|
|
.85 |
Variants |
9 |
4 |
|
Conventional |
51 |
20 |
|
Tumor multifocality |
|
|
.05 |
Yes |
26 |
5 |
|
No |
34 |
19 |
|
Tumor size (cm) |
|
|
.17 |
< 2 |
52 |
24 |
|
2-4 |
6 |
0 |
|
> 4 |
2 |
0 |
|
Lymphovascular invasion |
|
|
< .01 |
Yes |
12 |
12 |
|
No |
48 |
12 |
|
Extrathyroid extension |
|
|
< .01 |
Yes |
11 |
12 |
|
No |
49 |
12 |
|
Lymph node metastasis |
|
|
.47 |
Yes |
13 |
7 |
|
No |
47 |
17 |
|
Table 5.P2X7R expression in papillary thyroid cancer without Hashimoto's thyroiditis
Characteristic |
P2X7R (scores 0-2) (n = 20) |
P2X7R (scores 3-9) (n = 66) |
p-value |
Age at diagnosis (yr) |
|
|
< .01 |
< 45 |
19 |
11 |
|
≥ 45 |
1 |
55 |
|
Gender |
|
|
.74 |
Male |
3 |
12 |
|
Female |
17 |
54 |
|
Histological variant |
|
|
.55 |
Variants |
1 |
6 |
|
Conventional |
19 |
60 |
|
Tumor multifocality |
|
|
< .01 |
Yes |
0 |
34 |
|
No |
20 |
32 |
|
Tumor size (cm) |
|
|
.84 |
< 2 |
16 |
53 |
|
2-4 |
4 |
12 |
|
> 4 |
0 |
1 |
|
Lymphovascular invasion |
|
|
.48 |
Yes |
5 |
22 |
|
No |
15 |
44 |
|
Extrathyroid extension |
|
|
.13 |
Yes |
10 |
21 |
|
No |
10 |
45 |
|
Lymph node metastasis |
|
|
.94 |
Yes |
8 |
27 |
|
No |
12 |
39 |
|