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Ju Young Song 3 Articles
Construction of High-Density Tissue Microarrays at Low Cost by Using Self-Made Manual Microarray Kits and Recipient Paraffin Blocks
Chang Hwan Choi, Kyu Ho Kim, Ju Young Song, Suk Jin Choi, Lucia Kim, In Suh Park, Jee Young Han, Joon Mee Kim, Young Chae Chu
Korean J Pathol. 2012;46(6):562-568.   Published online December 26, 2012
DOI: https://doi.org/10.4132/KoreanJPathol.2012.46.6.562
  • 10,364 View
  • 98 Download
  • 14 Crossref
AbstractAbstract PDF
Background

Advances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived pathology specimens. However, the relatively high cost of TMA construction may hamper many researchers from using this essential tool of modern pathology research. We discuss methods for making TMA kits and recipient blocks for manual construction of high-density TMAs at low cost.

Methods

Ordinary cannula piercing needles, hypodermic needles, bone marrow biopsy needles, metallic ink cartridges of ballpoint pens, and disposable skin biopsy punches were used to construct self-made manual TMA kits. The recipient blocks were manufactured by boring holes in the conventional bare paraffin blocks. A mini electric hand drill and a microcompound table assembled on a drill stand were used to maximize the capacity of the recipient blocks.

Results

By using TMA kits made from cannula piercing needles (16- and 18-gauge), it was possible to construct TMAs with 1 mm×140 cores, 0.6 mm×320 cores, 2 mm×70 cores, 3 mm×35 cores, and 5 mm×12 cores. The capacity of the recipient blocks could be dramatically increased by drilling holes.

Conclusions

Construction of TMAs using self-made TMA kits is an inexpensive alternative to construction of TMAs using commercial devices.

Citations

Citations to this article as recorded by  
  • Diagnostic Potential of CD44, CD133, and VDR in Epithelial Ovarian Tumors: Association with Histopathology Parameters
    Ljubiša Jovanović, Branka Šošić-Jurjević, Anđa Ćirković, Sandra Dragičević, Branko Filipović, Svetlana Milenković, Stefan Dugalić, Miroslava Gojnić-Dugalić, Aleksandra Nikolić
    International Journal of Molecular Sciences.2025; 26(8): 3729.     CrossRef
  • Constructing high-density tissue microarrays with a novel method and a self-made tissue-arraying instrument
    Ping Qin, Liu Li, Li Zhao, Piaopiao Bian, Zhongtang Xiong
    Pathology - Research and Practice.2023; 245: 154430.     CrossRef
  • The correlation of PD-L1 expression in cytological and histological material of serous high-grade ovarian cancer
    Ljubiša Jovanović, Anđa Ćirković, Ljubinka Nikolić, Milena Jović, Darko Mikić, Svetlana Milenković, Radmila Janković
    Srpski medicinski casopis Lekarske komore.2023; 4(3): 246.     CrossRef
  • Expression of estrogen and progesterone receptors, HER2 protein and Ki-67 proliferation index in breast carcinoma in both tumor tissue and tissue microarray
    UP Hacısalihoğlu, MA Dogan
    Biotechnic & Histochemistry.2022; 97(4): 298.     CrossRef
  • PD-L1 Expression in High-Grade Serous and Clear Cell Ovarian Cancer
    Ljubiša Jovanović, Andja Ćirković, Milena Jović, Radmila Janković
    Indian Journal of Gynecologic Oncology.2022;[Epub]     CrossRef
  • PD-L1 Expression in Different Segments and Histological Types of Ovarian Cancer According to Lymphocytic Infiltrate
    Ljubiša Jovanović, Radmila Janković, Andja Ćirković, Milena Jović, Tijana Janjić, Slaviša Djuričić, Svetlana Milenković
    Medicina.2021; 57(12): 1309.     CrossRef
  • Optimization of Tissue Microarrays from Banked Human Formalin-Fixed Paraffin Embedded Tissues in the Cancer Research Setting
    Tammy Sexton, Gregory L. Kucera, Edward A. Levine, Kounosuke Watabe, Stacey S. O'Neill
    Biopreservation and Biobanking.2019; 17(5): 452.     CrossRef
  • Peripheral nerve sheath tumor invading the nasal cavities of a 6-year-old female Pointer dog
    Alessandra Sfacteria, Laura Perillo, Francesco Macrì, Giovanni Lanteri, Claudia Rifici, Giuseppe Mazzullo
    Veterinary Quarterly.2015; 35(3): 170.     CrossRef
  • High Quality Tissue Miniarray Technique Using a Conventional TV/Radio Telescopic Antenna
    Mohamed A. Elkablawy, Abdulkader M. Albasri
    Asian Pacific Journal of Cancer Prevention.2015; 16(3): 1129.     CrossRef
  • Overview on Techniques to Construct Tissue Arrays with Special Emphasis on Tissue Microarrays
    Ulrich Vogel
    Microarrays.2014; 3(2): 103.     CrossRef
  • Tissue Microarray
    Kathleen Barrette, Joost J. van den Oord, Marjan Garmyn
    Journal of Investigative Dermatology.2014; 134(9): 1.     CrossRef
  • Altered Expression of PTEN and Its Major Regulator MicroRNA-21 in Pulmonary Neuroendocrine Tumors
    Hyoun Wook Lee, Seung Yeon Ha, Mee Sook Roh
    Korean Journal of Pathology.2014; 48(1): 17.     CrossRef
  • Optimizing tissue microarray construction procedure to improve quality of sections
    Hua Chang, Diane Peluso, Sadiq Hussain, Michail Shipitsin, Peter Blume-Jensen
    Journal of Histotechnology.2014; 37(3): 95.     CrossRef
  • In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks
    Kyu Ho Kim, Suk Jin Choi, Yeon Il Choi, Lucia Kim, In Suh Park, Jee Young Han, Joon Mee Kim, Young Chae Chu
    Korean Journal of Pathology.2013; 47(3): 238.     CrossRef
Granular Cell Astrocytoma: Report of a Case
Kyu Ho Kim, Ju Young Song, Chang Hwan Choi, Lucia Kim, Suk Jin Choi, Jee Young Han, Joon Mee Kim, Young Chae Chu, In Suh Park
Korean J Pathol. 2012;46(4):370-372.   Published online August 23, 2012
DOI: https://doi.org/10.4132/KoreanJPathol.2012.46.4.370
  • 7,470 View
  • 70 Download
  • 4 Crossref
AbstractAbstract PDF

We report here a rare case of granular cell astrocytoma. A 75-year-old man was admitted to Inha University Hospital with a three-month history of language deterioration. In a magnetic resonance imaging, a 6.5 cm-sized heterogeneous enhancing mass was seen in both the frontal lobes and the anterior genu of the corpus callosum. A stereotactic biopsy was performed. The tumor was composed of large and small round cells with abundant intracytoplasmic granules. The nuclei were bland, round to oval, and often eccentrically located. The cytoplasm of the tumor cells was positive for glial fibrillary acidic protein and S-100 protein.

Citations

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  • Cytopathological findings of granular cell glioblastoma in intraoperative squash smear preparations: A case report
    Samuel López‐Muñoz, Borja Sánchez‐Cordon, Mario Taravilla‐Loma, Isabel Esteban‐Rodríguez
    Cytopathology.2025; 36(1): 85.     CrossRef
  • Granular cell tumor of the brain: case report and review of literature
    Shyam Duvuru, Vivek Sanker, Deepak Pandit, Sheezah Khan, Sara Alebrahim, Tirth Dave
    Journal of Surgical Case Reports.2023;[Epub]     CrossRef
  • Granular cell astrocytoma: Case report
    Rohan Gupta, Supriya Gupta, Nathaniel Shapiro, Scott Rahimi, Suash Sharma
    Human Pathology: Case Reports.2018; 14: 4.     CrossRef
  • Unusual presentation of a granular cell astrocytoma
    Alan A George, Gregory N Fuller, Lauren A Langford, Clayton D Simon, Amy A Zingalis, Derek A Mathis
    Histopathology.2013; 63(6): 883.     CrossRef
Adenocarcinoma with Intraductal Papillary Mucinous Neoplasm Arising in Jejunal Heterotopic Pancreas
Ju Young Song, Jee Young Han, Sun Keun Choi, Lucia Kim, Suk Jin Choi, In Suh Park, Young Chae Chu, Kyu Ho Kim, Joon Mee Kim
Korean J Pathol. 2012;46(1):96-100.   Published online February 23, 2012
DOI: https://doi.org/10.4132/KoreanJPathol.2012.46.1.96
  • 9,236 View
  • 62 Download
  • 14 Crossref
AbstractAbstract PDF

A 74-year-old man suffered from jejunal perforation and adhesion to sigmoid colon due to adenocarcinoma associated with intraductal papillary mucinous neoplasm (IPMN) arising in a jejunal heterotopic pancreas. The jejunal lesion showed direct extension to the sigmoid colon, which was mistaken as sigmoid colon cancer by surgeons. Malignant transformation is a rare complication of a heterotopic pancreas. About half of malignancies in reported cases were ductal adenocarcinoma arising in the stomach, and the jejunal location is extremely rare. Furthermore, IPMN is also uncommon finding in a heterotopic pancreas.

Citations

Citations to this article as recorded by  
  • Intraductal Papillary Mucinous Neoplasm Arising from Heterotopic Pancreas in Stomach: A Case Report and Review of Literature
    Yu Pang, Yuyu Liu, Qinqin Liu, Gang Hou
    International Journal of Surgical Pathology.2023; 31(5): 708.     CrossRef
  • Clinicopathological features of intraductal papillary mucinous neoplasm derived from ectopic pancreas: A systematic review
    Jiro Kimura, Takehiro Okabayashi, Kenta Sui, Takahiro Murokawa, Motoyasu Tabuchi, Masaki Aida, Jun Iwata, Yasuhiro Hata
    Surgery Open Science.2022; 8: 62.     CrossRef
  • Clinicopathological Features of Intraductal Papillary Mucinous Neoplasm Derived from Ectopic Pancreas: A Systematic Review
    Jiro Kimura, Takehiro Okabayashi, Kenta Sui, Takahiro Murokawa, Motoyasu Tabuchi, Masaki Aida, Jun Iwata, Yasuhiro Hata
    SSRN Electronic Journal .2022;[Epub]     CrossRef
  • A case of ectopic pancreas of the stomach accompanied by intraductal papillary mucinous neoplasm with GNAS mutation
    Naoko Nambu, Takashi Yamasaki, Nami Nakagomi, Tsutomu Kumamoto, Tatsuro Nakamura, Akio Tamura, Toshihiko Tomita, Hiroto Miwa, Hisashi Shinohara, Seiichi Hirota
    World Journal of Surgical Oncology.2021;[Epub]     CrossRef
  • Intraductal Papillary Mucinous Neoplasm Arising in a Heterotopic Pancreas Treated by Percutaneous Endoscopic Intragastric Surgery
    Shoko Moue, Yoshimasa Akashi, Koichi Ogawa, Katsuji Hisakura, Tsuyoshi Enomoto, Yusuke Ohara, Yohei Owada, Shingo Sakashita, Tatsuya Oda
    The Japanese Journal of Gastroenterological Surgery.2020; 53(4): 371.     CrossRef
  • Adenocarcinoma with intraductal papillary mucinous neoplasm arising in a duodenal heterotopic pancreas: a case report
    Etsuko Hisanaga, Takaaki Sano, Norio Kubo, Norihiro Ishii, Ken Shirabe, Hitoshi Takagi, Junko Hirato, Hayato Ikota
    Clinical Journal of Gastroenterology.2020; 13(6): 1373.     CrossRef
  • Malignant Transformation of Ectopic Pancreas
    Irina M. Cazacu, Adriana Alexandra Luzuriaga Chavez, Graciela M. Nogueras Gonzalez, Adrian Saftoiu, Manoop S. Bhutani
    Digestive Diseases and Sciences.2019; 64(3): 655.     CrossRef
  • Anomalien im pankreatikobiliären System
    Dirk Walter, Michael Jung
    Der Gastroenterologe.2019; 14(4): 301.     CrossRef
  • Intraductal papillary mucinous neoplasm originating from a heterotopic pancreas within the jejunum: a case report
    Eiji Noda, Kenji Kuroda, Tomohisa Sera, Takuya Mori, Haruhito Kinoshita, Tsuyoshi Hasegawa, Hitoshi Teraoka, Takaaki Chikugo
    Journal of Surgical Case Reports.2018;[Epub]     CrossRef
  • Heterotopic Pancreas of the Gastrointestinal Tract and Associated Precursor and Cancerous Lesions
    Sun-Young Jun, Dahye Son, Mi-Ju Kim, Sung Joo Kim, Soyeon An, Young Soo Park, Sook Ryun Park, Kee Don Choi, Hwoon-Yong Jung, Song Cheol Kim, Jeong Hwan Yook, Byung-Sik Kim, Seung-Mo Hong
    American Journal of Surgical Pathology.2017; 41(6): 833.     CrossRef
  • Heterotopic Pancreas within the Proximal Hepatic Duct, Containing Intraductal Papillary Mucinous Neoplasm
    Alistair J. Lawrence, Aducio Thiessen, Amy Morse, A. M. James Shapiro
    Case Reports in Surgery.2015; 2015: 1.     CrossRef
  • Bowel Rest
    Payal Saxena, Deborah Belchis, Anne Marie Lennon
    Clinical Gastroenterology and Hepatology.2015; 13(13): e173.     CrossRef
  • Adenocarcinoma arising from jejunal ectopic pancreas mimicking peritoneal metastasis from colon cancer: a case report and literature review
    Yusuke Yamaoka, Tomohiro Yamaguchi, Yusuke Kinugasa, Akio Shiomi, Hiroyasu Kagawa, Yushi Yamakawa, Masakatsu Numata, Shinya Sugimoto, Kenichiro Imai, Kinichi Hotta, Keiko Sasaki
    Surgical Case Reports.2015;[Epub]     CrossRef
  • Intraductal papillary mucinous neoplasm of the ileal heterotopic pancreas in a patient with hereditary non-polyposis colorectal cancer: A case report
    Sang Hwa Lee
    World Journal of Gastroenterology.2015; 21(25): 7916.     CrossRef

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