- Construction of High-Density Tissue Microarrays at Low Cost by Using Self-Made Manual Microarray Kits and Recipient Paraffin Blocks
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Chang Hwan Choi, Kyu Ho Kim, Ju Young Song, Suk Jin Choi, Lucia Kim, In Suh Park, Jee Young Han, Joon Mee Kim, Young Chae Chu
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Korean J Pathol. 2012;46(6):562-568. Published online December 26, 2012
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DOI: https://doi.org/10.4132/KoreanJPathol.2012.46.6.562
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10,364
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- Background
Advances of tissue microarray (TMA) technology have enabled simultaneous in situ analysis of biomarker expression in a large number of archived pathology specimens. However, the relatively high cost of TMA construction may hamper many researchers from using this essential tool of modern pathology research. We discuss methods for making TMA kits and recipient blocks for manual construction of high-density TMAs at low cost. MethodsOrdinary cannula piercing needles, hypodermic needles, bone marrow biopsy needles, metallic ink cartridges of ballpoint pens, and disposable skin biopsy punches were used to construct self-made manual TMA kits. The recipient blocks were manufactured by boring holes in the conventional bare paraffin blocks. A mini electric hand drill and a microcompound table assembled on a drill stand were used to maximize the capacity of the recipient blocks. ResultsBy using TMA kits made from cannula piercing needles (16- and 18-gauge), it was possible to construct TMAs with 1 mm×140 cores, 0.6 mm×320 cores, 2 mm×70 cores, 3 mm×35 cores, and 5 mm×12 cores. The capacity of the recipient blocks could be dramatically increased by drilling holes. ConclusionsConstruction of TMAs using self-made TMA kits is an inexpensive alternative to construction of TMAs using commercial devices.
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Citations
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- Diagnostic Potential of CD44, CD133, and VDR in Epithelial Ovarian Tumors: Association with Histopathology Parameters
Ljubiša Jovanović, Branka Šošić-Jurjević, Anđa Ćirković, Sandra Dragičević, Branko Filipović, Svetlana Milenković, Stefan Dugalić, Miroslava Gojnić-Dugalić, Aleksandra Nikolić International Journal of Molecular Sciences.2025; 26(8): 3729. CrossRef - Constructing high-density tissue microarrays with a novel method and a self-made tissue-arraying instrument
Ping Qin, Liu Li, Li Zhao, Piaopiao Bian, Zhongtang Xiong Pathology - Research and Practice.2023; 245: 154430. CrossRef - The correlation of PD-L1 expression in cytological and histological material of serous high-grade ovarian cancer
Ljubiša Jovanović, Anđa Ćirković, Ljubinka Nikolić, Milena Jović, Darko Mikić, Svetlana Milenković, Radmila Janković Srpski medicinski casopis Lekarske komore.2023; 4(3): 246. CrossRef - Expression of estrogen and progesterone receptors, HER2 protein and Ki-67 proliferation index in breast carcinoma in both tumor tissue and tissue microarray
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- Granular Cell Astrocytoma: Report of a Case
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Kyu Ho Kim, Ju Young Song, Chang Hwan Choi, Lucia Kim, Suk Jin Choi, Jee Young Han, Joon Mee Kim, Young Chae Chu, In Suh Park
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Korean J Pathol. 2012;46(4):370-372. Published online August 23, 2012
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DOI: https://doi.org/10.4132/KoreanJPathol.2012.46.4.370
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7,470
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We report here a rare case of granular cell astrocytoma. A 75-year-old man was admitted to Inha University Hospital with a three-month history of language deterioration. In a magnetic resonance imaging, a 6.5 cm-sized heterogeneous enhancing mass was seen in both the frontal lobes and the anterior genu of the corpus callosum. A stereotactic biopsy was performed. The tumor was composed of large and small round cells with abundant intracytoplasmic granules. The nuclei were bland, round to oval, and often eccentrically located. The cytoplasm of the tumor cells was positive for glial fibrillary acidic protein and S-100 protein.
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- Adenocarcinoma with Intraductal Papillary Mucinous Neoplasm Arising in Jejunal Heterotopic Pancreas
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Ju Young Song, Jee Young Han, Sun Keun Choi, Lucia Kim, Suk Jin Choi, In Suh Park, Young Chae Chu, Kyu Ho Kim, Joon Mee Kim
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Korean J Pathol. 2012;46(1):96-100. Published online February 23, 2012
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DOI: https://doi.org/10.4132/KoreanJPathol.2012.46.1.96
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9,236
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62
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A 74-year-old man suffered from jejunal perforation and adhesion to sigmoid colon due to adenocarcinoma associated with intraductal papillary mucinous neoplasm (IPMN) arising in a jejunal heterotopic pancreas. The jejunal lesion showed direct extension to the sigmoid colon, which was mistaken as sigmoid colon cancer by surgeons. Malignant transformation is a rare complication of a heterotopic pancreas. About half of malignancies in reported cases were ductal adenocarcinoma arising in the stomach, and the jejunal location is extremely rare. Furthermore, IPMN is also uncommon finding in a heterotopic pancreas.
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