- Comparison of the DNA Preservation in Neutral-Buffered Formalin Fixed Paraffin-Embedded Tissue and in Non-Buffered Formalin Fixed Paraffin-Embedded Tissue.
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An Na Seo, Jae Hoon Kim, Dakeun Lee, Ji Yun Jeong, Ji Young Park
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Korean J Pathol. 2011;45(6):549-556.
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DOI: https://doi.org/10.4132/KoreanJPathol.2011.45.6.549
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 Abstract
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- BACKGROUND
The preservation of optimized DNA and its extraction from formalin-fixed, paraffin-embedded (FFPE) tissues are important issues. There has been some doubt over whether 10% neutral-buffered formalin is an ideal fixation solution for DNA preservation over non-buffered formalin, as conventionally recommended. In this study, the correlation between the efficiency of DNA extraction from FFPE tissues and buffered formalin was evaluated.
METHODS
Several tissues with same conditions except fixatives were fixed in four different formalin solution groups and were routinely processed as paraffin-embedding protocols. DNAs were extracted from four different FFPE tissues that were stored for over 3 months and over 9 months. The quantity and quality of the DNAs were assessed with a NanoDrop ND-1000 spectrophotometer, and the polymerase chain reaction (PCR) amplification and degradation were analyzed via microchip electrophoresis.
KRAS mutation analysis and microsatellite instability (BAT25) PCR were performed with each sample.
RESULTS
The results showed no remarkable difference in the four groups.
CONCLUSIONS
The study findings demonstrate that DNA preservation is fairly unaffected by a neutral buffer where there is short formalin manufacture period and an adequate formalin fixation time before embedding in paraffin.
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Citations
Citations to this article as recorded by  - Comparison of Direct Sequencing, PNA Clamping-Real Time Polymerase Chain Reaction, and Pyrosequencing Methods for the Detection ofEGFRMutations in Non-small Cell Lung Carcinoma and the Correlation with Clinical Responses to EGFR Tyrosine Kinase Inhibitor
Hyun Ju Lee, Xianhua Xu, Hyojin Kim, Yan Jin, Pingli Sun, Ji Eun Kim, Jin-Haeng Chung
Korean Journal of Pathology.2013; 47(1): 52. CrossRef
- Cytologic Distinctive Features of Brenner Tumor.
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Jung Sik Jang, An Na Seo, Seon Jae Lee, Ji Young Park
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Korean J Pathol. 2011;45(2):223-226.
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DOI: https://doi.org/10.4132/KoreanJPathol.2011.45.2.223
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- Herein, we present two cases of Brenner tumor, a rarely occurring neoplasm in the ovaries, obtained via intraoperative fine needle aspiration. The borderline Brenner tumor exhibited marked squamous metaplasia, characterized by individually distributed atypical squamous cells. A benign Brenner tumor associated with mucinous cystadenoma evidenced typical mucinous metaplastic features and transitional foci. These distinctive features may prove helpful in differential diagnosis of varied ovarian tumors, and particularly for intraoperative consultation.
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Citations
Citations to this article as recorded by - Pre‐operative cytodiagnosis of an adult granulosa cell tumour: report of a case with its differential diagnosis
S. R. Jinkala, S. E. Jacob, S. Neelaiah, B. A. Badhe
Cytopathology.2014; 25(1): 63. CrossRef
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