Hornychova et al. (2008) [41] |
Single institute |
Retrospective |
Anthracycline-taxane-based regimens |
IHC |
CD3 |
iTIL |
73 |
ypT0/Tis |
CD3+iTIL (p = .004, univariate) |
ypN0 |
Denkert et al. (2010) [42] |
GeparDuo |
Prospective |
EC-Doc (GeparDuo) |
H&E |
TILs |
sTIL |
1,058 |
ypT0/Tis |
sTILs (continuous, per 10% increase) (p = .001, multivariate) |
GeparTrio |
TAC±vinorBlbine/capecitabine (GeparTrio) |
iTIL |
ypN0 |
LPBC (binary ≥ 60%) (p = .001, multivariate) |
Denkert et al. (2015) [51] |
Geparsixto |
Prospective |
Anthracycline-taxane plus carbioplatin vs Anthracycline-taxane |
H&E |
TILs |
sTIL |
580 |
ypT0/Tis |
sTILs (continuous, per 10% increase) (p = .001, multivariate) |
mRNA assay |
12 Immune mRNA markers |
iTIL |
481 |
ypN0 |
12 Immune mRNA markers were predictive for increased pCR |
West et al. (2011) [44] |
Public gene expression data from EORTC 10994/BIG00-01 |
Prospective |
FEC vs TET |
Gene expression data |
Gene expression data |
Not associated |
99 |
Undefined |
High TIL signature correlate with pCR (p = .001, multivariate) |
Ono et al. (2012) [52] |
Single institute |
Retrospective |
Anthracycline-based; cyclophosphamide-based or taxane-based regimens |
H&E |
TILs |
sTIL |
92 TNBC |
ypT0 |
Total TILs correlate with pCR (p = .015, multivariate) |
iTIL |
Yamaguchi et al. (2012) [53] |
Single institute |
Retrospective |
Anthracycline-taxane-based regimens |
H&E |
TILs |
Total TILs |
68 |
ypT0/Tis |
Total TILs correlate with pCR (p<.001, multivariate) |
Oda et al. (2012) [54] |
Institutional |
Retrospective |
Paclitaxel followed by FEC |
IHC |
CD8, FOXP3, IL17F |
iTIL |
180 |
ypT0ypN0 |
FOXP3 positively correlated with pCR (p = .014, multivariate) |
Schmidt et al. (2012) [35] |
Public gene expression data (7 cohorts) |
Retrospective |
Anthracycline-based regimen |
Gene expression data |
Gene expression data |
Not associated |
845 |
Undefined |
IGKC positively correlated with pCR (p<.001) |
Issa-Nummer et al. (2013) [55] |
GeparQuinto Predict study |
EC-D |
EC followed by D |
H&E |
TILs |
sTIL |
313 HER2– |
ypT0ypN0 |
sTILs and LPBC associated with pCR (p = .01, multivariate) |
iTIL |
LPBC associated with pCR (p = .003, multivariate) |
Seo et al. (2013) [25] |
Single institute |
Retrospective |
Anthracycline-taxane-based regimens |
IHC |
CD8, CD4, FOXP3 |
iTILs |
153 |
ypT0ypN0 |
CD8 positively correlated with pCR (p = .003, multivariate) |
Lee et al. (2013) [56] |
Single institute |
Retrospective |
Anthracycline-taxane-based regimens |
H&E |
TILs, CD3, CD8, FOXP3 |
sTILs |
175 |
ypT0ypN0 |
sTIL positively correlated with pCR (p = .024, multivariate) |
IHC |
Nabholtz et al. (2014) [57] |
Phase II TVA study |
Prospective |
FEC with/without D with panitumumab |
IHC |
CD8 |
Not associated |
47 |
ypT0/Tis |
CD8 positively correlated with pCR (p = .000003, univariate) |
ypN0 |