Fig. 1Distribution of high-risk human papillomavirus (HPV) infections.
Fig. 2Laser scanning results of human papillomavirus (HPV) 52 and 58 genotypes using HPV DNA chip. (A) Format of HPV DNA chip (M, position marker). (B) HPV negative. (C) HPV 52 positive. (D) HPV 58 positive.
Fig. 3Immunohistochemical studies for p16 (A, B) and p53 (C, D) in cervical intraepithelial neoplasia (CIN); positive nuclear and cytoplasmic reactions for p16 (A, CIN II; B, CIN III) and positive nuclear reactions for p53 (C, CIN II; D, CIN III).
Table 1.Distribution of HPV 52 and 58 genotypes according to histologic grades in 62 cervical tissues
HPV genotypes |
Histology
|
WNL |
CIN I |
CIN II/III |
CIS |
Total |
HPV 52 (single) |
8 |
6 |
3 |
2 |
19 |
HPV 58 (single) |
3 |
8 |
10 |
2 |
23 |
HPV 52 (multiple) |
1 |
2 |
0 |
1 |
4 |
HPV 58 (multiple) |
5 |
4 |
4 |
3 |
16 |
Total |
17 |
20 |
17 |
8 |
62 |
Table 2.Expression of p16 and p53 proteins in 62 cervical tissues infected with HPV 52 and 58
Histology |
p16 expression
|
p-value |
p53 expression
|
p-value |
Negative |
Positive |
Negative |
Positive |
WNL |
7 (41.2) |
10 (58.8) |
.47 |
11 (64.7) |
6 (35.3) |
.80 |
CIN I |
8 (40.0) |
12 (60.0) |
|
11 (55.0) |
9 (45.0) |
|
CIN II/III |
5 (29.4) |
12 (70.6) |
|
12 (70.6) |
5 (29.4) |
|
CIS |
1 (12.5) |
7 (87.5) |
|
5 (62.5) |
3 (37.5) |
|
Total |
21 (33.9) |
41 (66.1) |
|
39 (62.9) |
23 (37.1) |
|
Table 3.Relationship between expression of p16 and p53 protein in 45 cervical intraepithelial neoplasias infected with HPV 52 and 58
Expression |
p53 protein
|
p-value |
Negative |
Positive |
p16 protein |
|
|
.42 |
Negative |
7 (25.0) |
7 (41.2) |
|
Positive |
21 (75.0) |
10 (58.8) |
|