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Original Article
- The Significance of TROP2 Expression in Predicting BRAF Mutations in Papillary Thyroid Carcinoma
- Joon Seog Kong, Hyeon Jin Kim, Min-Jung Kim, Areumnuri Kim, Dalnim Lee, Kanghee Han, Sunhoo Park, Jae Soo Koh, Jae Kyung Myung
- J Pathol Transl Med. 2018;52(1):14-20. Published online December 11, 2017
- DOI: https://doi.org/10.4132/jptm.2017.10.17
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- 9 Web of Science
- 9 Crossref
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Abstract
PDF - Background
Trophoblast antigen 2 (TROP2) is a human trophoblast cell-surface glycoprotein that is overexpressed in several types of epithelial cancers, and is suggested to be associated with an unfavorable prognosis. BRAF mutations are the most common genetic alteration in papillary thyroid carcinoma (PTC). We evaluated the correlation between TROP2 expression and BRAF mutation in PTC.
Methods
First, we carried out pyrosequencing for BRAF mutations and immunohistochemistry for TROP2 expression with a tissue microarray consisting of 52 PTC cases. Membranous staining in at least 5% of tumor cells was designated as positive staining and we analyzed the relationship between TROP2 expression and diverse clinicopathological factors, including BRAF mutation. Second, we tested TROP2 mRNA expression in three thyroid cancer cell lines with BRAF mutations (BCPAP, SNU790, and 8505C) and a normal thyroid cell line. Additionally, we checked TROP2 protein levels in a normal thyroid cell line after introduction of the BRAF V600E mutation.
Results
In this study, 21 of 26 cases with BRAF mutation showed TROP2 immunoreactivity, whereas all 26 cases without BRAF mutation showed no immunoreactivity for TROP2 with a statistically significant difference (p<.001). Upregulation of TROP2 mRNA was observed in all three thyroid cancer cell lines, but not in the normal thyroid cell line. Interestingly, however, the TROP2 expression was increased in the normal thyroid cell line after introduction of the BRAF V600E mutation.
Conclusions
Based on these results, we concluded that TROP2 expression is significantly associated with BRAF mutation and that TROP2 immunohistochemistry could be used for predicting BRAF mutations or diagnosing papillary thyroid carcinoma. -
Citations
Citations to this article as recorded by
- Diagnostic and prognostic utility of TROP-2, SLP-2, and CXCL12 expression in papillary thyroid carcinoma
Amany Selim Attia, Samia Hussein, Hend Sameh, Amr Khalil, Ahmad Barakat Waley, Ihab Matar, Reham Sameh
Cancer Biomarkers.2024; 39(3): 211. CrossRef - TROP2 is a Good Indicator for Infiltrative Nature of Carcinoma Rather than Diagnosing Malignancy in Thyroid
E. Kılınc, P. Gunes, A. Doganer
Indian Journal of Otolaryngology and Head & Neck Surgery.2022; 74(S2): 2560. CrossRef - Advances in Trop2-targeted therapy: Novel agents and opportunities beyond breast cancer
Xinlin Liu, Junwen Deng, Yang Yuan, Wujun Chen, Wenshe Sun, Yanhong Wang, Haiming Huang, Bing Liang, Tao Ming, Jialian Wen, Binghuan Huang, Dongming Xing
Pharmacology & Therapeutics.2022; 239: 108296. CrossRef - Knockdown of Trop2 inhibits proliferation and migration and induces apoptosis of endometrial cancer cells via AKT/β‐catenin pathway
Xiaotong Sun, Guangyang Xing, Cui Zhang, Kun Lu, Yuqiong Wang, Xiyan He
Cell Biochemistry and Function.2020; 38(2): 141. CrossRef - Can TROP2 be used as a prognostic marker in endometrioid endometrial carcinoma?
SerkanY Çelik, Özgürİlhan Çelik
Indian Journal of Pathology and Microbiology.2020; 63(3): 418. CrossRef - Diagnostic Value of TROP-2 and CK19 Expression in Papillary Thyroid Carcinoma in Both Surgical and Cytological Specimens
Asmaa Gaber Abdou, Mohammed Shabaan, Rania Abdallha, Nehal Nabil
Clinical Pathology.2019; 12: 2632010X1986304. CrossRef - Evaluation of Diagnostic Utility of Immunohistochemistry Markers of TROP-2 and HBME-1 in the Diagnosis of Thyroid Carcinoma
Nooshin Zargari, Maral Mokhtari
European Thyroid Journal.2019; 8(1): 1. CrossRef - The diagnostic value of TROP-2, SLP-2 and CD56 expression in papillary thyroid carcinoma
Xueyang Yang, Yifang Hu, He Shi, Chengzhou Zhang, Zhixiao Wang, Xiaoyun Liu, Huanhuan Chen, Lijuan Zhang, Dai Cui
European Archives of Oto-Rhino-Laryngology.2018; 275(8): 2127. CrossRef - TROP2 promotes cell proliferation and migration in osteosarcoma through PI3K/AKT signaling
Qing‑Zhi Gu, Abulimiti Nijiati, Xing Gao, Kai‑Liang Tao, Cheng‑Duo Li, Xue‑Peng Fan, Zheng Tian
Molecular Medicine Reports.2018;[Epub] CrossRef
- Diagnostic and prognostic utility of TROP-2, SLP-2, and CXCL12 expression in papillary thyroid carcinoma
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