MATERIALS AND METHODS

We reviewed 27 endomyocardial biopsies and three whole hearts after cardiac transplantation from 25 patients at Seoul National University Hospital from 2012 to May 2017. Cases were collected from those with a clinical diagnosis of cardiac sarcoidosis when endomyocardial biopsy was performed. Clinical suspicion of cardiac sarcoidosis was made when there is an arrhythmia and extracardiac sarcoidosis; or certain types of arrhythmia (the second degree Mobitz type II or the third-degree atrioventricular block with or without radiologic features suggestive of cardiac sarcoidosis). We included consecutive cases with different levels of probability suspected by the physician. Clinical summary of 27 biopsies from 25 patients are described in Table 1.

Interpretation criteria of magnetic resonance imaging

The magnetic resonance imaging (MRI) findings were categorized as follows: (1) probable cases: these cases involve multiple patchy enhancement at the subepicardial or mid-myocardial layers, which do not correspond to the expected territories of coronary arterial supply. Involvement of the basal part of the interventricular septum, irregular thinning of the wall, and ejection fraction less than 50% are considered probable cases; (2) possible cases: these cases have features similar to those of probable cases, but diseases other than cardiac sarcoidosis could be considered a cause of the lesion; (3) nonspecific findings: these cases have minor and focal lesions of fibrosis, which may be interpreted as early lesions of sarcoidosis or other conditions; and (4) unlikely: these cases have radiological features more likely to indicate an ischemic condition or other specific conditions.

Interpretation criteria of four pathologic findings

The endomyocardial biopsies were stained with hematoxylin and eosin stain, Masson’s trichrome (MT) stain, and immunohistochemical stain for CD68. The pathological findings were analyzed based on four morphological changes: micro-granulomas, increased histiocytes, confluent fibrosis, and fatty tissue. Subsequently, cases were described and analyzed according to groups of morphologic findings.

Micro-granuloma

A granuloma is defined as a collection of histiocytes. The size of granulomas vary and there is no consensus on the minimum size of granuloma, although it is generally accepted that the granuloma has a size more than 30 or 50 histiocytes. In this study, we interpreted a small nodular collection of 5–10 histiocytes as a micro-granuloma. CD68 staining was necessary to find a micro-granuloma (Fig. 1).

Increased histiocytes

In some cases, infiltration of histiocytes was observed at the myocardial interstitium. We classified a significant increase in histiocytes in the interstitium when histiocytes are at least three times more than their usual frequency. CD68 immunostaining was necessary to count the number of histiocytes.

Confluent fibrosis

Confluent fibrosis replacing more than 30–50 myocardial cells was considered as a significant post-necrotic lesion in this study. The fibrotic lesion was more or less edematous and was associated with some inflammatory cells. We did not count the slender collagen fibers in the interstitium without myocyte damage, which was commonly encountered as interstitial fibrosis. Multifocal spotty fibrosis following necrosis of individual myocardial cells (replacement fibrosis) was also excluded in this study. MT stain was useful to reveal confluent fibrosis.

Fatty change

Post-inflammatory infiltrated fat cells were also recognized as individual fat cells in the fibrotic myocardial scar. Subendocardial accumulation of fat cells was also observed. Sometimes young fat cells with small and bubbly cytoplasm were noted. Differentiating them from fatty tissue as a normal component of the interstitium between myocardial bundles and perivascular space or as an extension of epicardial fatty tissue was difficult. Therefore, we noted infiltrations of fatty tissue of more than 10 adipocytes, which were interpreted as the presence of fatty tissue in this study.

Four cases received cardiac transplantation and three transplant hearts were examined by conventional gross dissection of the heart. A semi-quantitative histological mapping was performed on a sectional plane of a heart. Pathologic features were examined to determine granulomas and other related pathologic findings. An autopsied heart was examined, and microscopic images were added as supplementary material to compare classical sarcoidosis before treatment with our clinical cases in endomyocardial biopsies and heart transplantation.

RESULTS

Histopathologic findings of 27 endomyocardial biopsies

Endomyocardial biopsies with micro-granuloma as well as histiocytic infiltration, confluent fibrosis, and fatty change

Five endomyocardial biopsies were included in group 1 (Table 1). None of the five endomyocardial biopsies were interpreted as granuloma in the initial pathologic report because of small atypical granuloma-like collections of 5–10 histiocytes. We interpreted these cases as micro-granulomas based on the current definition for this study. One case was reported as myocarditis and four others had confluent fibrosis associated with increased histiocytes. One case was the second endomyocardial biopsy after the first biopsy showed no micro-granulomas two years previously. No cases had any evidence of extracardiac sarcoidosis. All five cases showed confluent fibrosis, increased histiocytes, and fatty change (Fig. 1). Two cases received cardiac transplantation after which the resected hearts showed granulomas (Fig. 2).

Four of these patients had MRI findings indicating probable sarcoidosis. One case was not checked for cardiac MRI but clinical findings were suggestive of cardiac sarcoidosis; global hypokinesia on the echocardiography and low left ventricular ejection fraction (30%) without any evidence of ischemic heart disease.

Endomyocardial biopsies with histiocytic infiltration, confluent fibrosis, and fatty change but without micro-granuloma

Two endomyocardial biopsies from a patient (group 2) showed an increase in histiocytes, confluent fibrosis, and fatty change. Two endomyocardial biopsies showed confluent fibrosis admixed with fatty change and increased histiocytes in the same area within the biopsied tissue (Fig. 3). Granuloma was not definitive but nodules of 3–5 histiocytes were scattered. MRI findings were compatible with sarcoidosis, although extracardiac evidence of sarcoidosis was lacking.

Endomyocardial biopsies with confluent fibrosis associated with fatty tissue infiltration

Confluent fibrosis was associated with fatty infiltration in six cases (group 3). Fatty change was observed at the immediate subendocardium or in the fibrous scar. Adipocytes showed variable sizes and were admixed with fibrosis, particularly at the margin of the fatty tissue and at the junction of the myocardium (Fig. 4).

One case was associated with extracardiac sarcoidosis in the lungs and mediastinal lymph nodes. MRI findings of four cases were compatible with sarcoidosis, though one case was excluded. MRI was not performed in two cases. The echocardiographic results of two cases showed unexplained ventricular dysfunction (left ventricular ejection fraction of 33%) with global hypokinesia and basal septal akinesia with thinning, respectively, which suggested cardiac sarcoidosis.

Presence of confluent fibrosis without associated fatty tissue

Three cases in group 4 showed confluent fibrosis (Fig. 5). Two cases showed hyalinized fibrosis, and one case showed fibrosis and edematous stroma. Confluent fibrosis is not evident in a small biopsy, but fibrosis larger than five times the diameter of myocardial cells can be interpreted as a scar related to a previous site of granuloma. Perivascular interstitium sometimes expanded to mimic confluent fibrosis and a fibrotic lesion around the blood vessels should be excluded before interpreting post-granuloma scar. Confluent fibrosis may be young edematous lesion, but may also be a hyalinized scar.

Presence of fatty tissue without associated fibrosis

Six cases in group 5 showed fatty tissue without associated fibrosis. Fatty tissue was an expansion of perivascular fatty tissue or fatty tissue between myocardial muscle groups (Fig. 4D).

One case showed MRI findings consistent with sarcoidosis, whereas three cases showed MRI findings not consistent with sarcoidosis. Two other cases had sarcoidosis in the mediastinal lymph node or skin, but the MRI findings did not support sarcoidosis.

None of four possible indicators on endomyocardial biopsy

Six cases showed normal myocardium or only minimal interstitial fibrosis (group 6). Three cases showed nonspecific findings on MRI. Two cases showed unlikely diagnosis on MRI. One case did not have MRI results, but the echocardiography showed normal left ventricular function with unexplained basal septal akinesia with thinning, because of which cardiac sarcoidosis was suspected.

Pathology of three cases of cardiac transplantation and an autopsy case

One case was clinically diagnosed as cardiac sarcoidosis with dilated right ventricular chamber. The initial pathological diagnosis of endomyocardial biopsy in this >40 years old man was myocarditis possibly related to systemic lupus erythematosus. He was treated with cyclophosphamide, methylon, cyclosporine A, and azathioprine. MRI findings were compatible with sarcoidosis (Supplementary Fig. S1).

Review of endomyocardial biopsy revealed three foci of a very small collection of 8–10 histiocytes, which was not interpreted as a granuloma at the initial pathologic examination (Fig. 1A).

The resected heart weighed 350 g. There were multiple white scars on the myocardium (Fig. 6). The right ventricular free wall was severely fibrotic and no myocardial tissue was seen at some parts of the right ventricular wall. The trabeculae at the right ventricular apex were severely atrophic and only fibrous cords were noted (Supplementary Fig. S2). An entire plane of the shortaxis of the heart showed large scars scattered at the left and right ventricles and ventricular septum (Fig. 6B). The distribution of the scars was comparable to that of multifocal subendocardial enhancement by cardiac MRI (Supplementary Fig. S1). In the multiple myocardial scars, scattered histiocytes and a few micro-granulomas were found (Supplementary Fig. S2).

The second case had hypertrophied right and left ventricles with multifocal fibrosis.

The initial clinical diagnosis of this >40 years old man was cardiac amyloidosis. Echocardiography showed a borderline increase in the left ventricular wall thickness with apical regional wall motion abnormalities. Cardiac MRI showed patchy, irregular, and delayed enhancement sparing subendocardium suggesting non-ischemic cardiomyopathy (Supplementary Fig. S3). PET with 2-deoxy-2-[¹⁸F]fluoro- D-glucose (FDG-PET) did not show hot uptake in the myocardium. Holter monitoring showed non-sustained ventricular tachycardia of three beats on two occasions.

Endomyocardial biopsy was performed twice at ages 49 and 51. One biopsy at age 51 showed focal patchy fibrosis with a single micro-granuloma (Supplementary Fig. S4).

The resected heart after transplantation at age 51 weighed 560 g. Both right and left ventricular walls were hypertrophied. There were multiple white scars in the myocardium, particularly in the left ventricle, the ventricular septum, and papillary muscles (Fig. 7). Histologically, the scars were dense fibrous scars and had fatty replacement. At least five micro-granulomas and histiocytes were scattered (Fig. 2).

The third case was clinically suspected as cardiac sarcoidosis but pathological study denied the diagnosis after examination of the resected heart. The initial clinical presentation of a >40 years old man was dyspnea on exertion and edema in the lower extremities. Complete atrioventricular block was noted, and a permanent pacemaker was inserted. Heart transplantation was performed at age 45. MRI was not performed, but ¹⁸F-FDG-PET revealed a localized hypermetabolic lesion at the apex and apical and midinferior anteroseptal wall. Computed tomography angiography showed no significant myocardial fibrosis.

The endomyocardial biopsy revealed three large pieces with different features. One piece showed confluent fibrosis and focal fatty infiltration. The second and third pieces were relatively well-preserved myocardium with interstitial fibrosis.

The resected heart weighed 365 g and both ventricles were enlarged. There was multifocal fibrosis in the left ventricular wall and septum. The mid-septal fibrosis was prominent and was diffuse rather than patchy. Fatty infiltration was seen at the anterior end of the basal part of the ventricular septum (Fig. 8). The fibrosis pattern was diffuse in the myocardium, but dense hyalinized fibrosis was seen in the mid-septal area (Fig. 8). We interpreted this explant heart as non-specified dilated cardiomyopathy rather than sarcoidosis.

An autopsy case (a man who died suddenly and a heart with many classical granulomas)

We reviewed a previously reported autopsied heart with sarcoidosis [19]. Macroscopic findings of the classical cardiac sarcoid lesion and histologic details are enclosed as supplementary materials. This 43-year-old man presented with syncope without any significant clinical history. Forensic autopsy revealed enlarged lymph nodes with sarcoid granuloma. The heart weighed 490 g and there were several conglomerated mass-like lesions (5.0× 1.2 cm) at the anterior wall of the left ventricle adjacent to the left anterior descending coronary artery (Supplementary Fig. S5). Histological examination showed active non-caseating granulomata involving multiple sites in the left and right ventricles. Lesions were subepicardial, subendocardial, or transmural in the ventricular wall (Supplementary Fig. S6). Fatty infiltration or fatty replacement was also noted even in the deep myocardium. Immunohistochemistry revealed CD68-positive histiocytes and CD3-positive T-lymphocytes (Supplementary Fig. S7).

DISCUSSION

Presence of classical granuloma in cardiac tissue is considered as the gold standard for diagnosis of sarcoidosis although infectious and immunologic causes are suggested in some cases [20,21]. Pathologists are asked to report the presence or absence of granulomas in small endocardial biopsies, and granulomas are not seen in most biopsies. When we don’t see any granulomas, it would be more useful for clinicians whether the case has some indirect or suggestive finding, rather than excluding the case from pathological diagnosis of sarcoidosis. We believe four histological findings and their combinations will have some value for pathological support of clinical practice on patients with cardiac sarcoidosis.

We compared our findings with radiological findings (Table 2). The presence of micro-granulomas (four cases, excluding a case without MRI findings) or increased histiocytic infiltration (two biopsies from one patient) was always (100%) associated with fatty infiltration and confluent fibrosis, and these cases showed radiological features of probable sarcoidosis. Among six cases with fatty change and confluent fibrosis, five cases had MRI findings. The MRI findings of three cases indicated probable cardiac sarcoidosis (3/5 or 60% possibility of sarcoidosis by radiology). When a single finding of either confluent fibrosis or fatty change was present, three of nine cases (33%) radiologically supported sarcoidosis. From these observations, we can categorize patients with two (fatty change and confluent fibrosis) or more indicators as cases with probable sarcoidosis on endomyocardial biopsy.

Clinical findings were so diverse that they matched poorly with pathological or radiological findings. None of the six cases with confirmed granulomas or increased histiocytes showed heart block. Five among 12 cases (42%) with pathological features of more than two indicators (confluent fibrosis and fatty change) had heart block. There were seven cases with heart block and five of them (71%) were probable for cardiac sarcoidosis by our pathological criteria.

In regard to corticosteroid treatment on cardiac sarcoidosis, cases at early inflammatory phase will get more benefit than cases with end-stage fibrotic lesions [22,23]. The detection of active definitive granulomas would be a definitive indication for steroid treatment. It is not surprising therefore that there was a poor correlation with the pathological parameters in this series.

The non-caseating granuloma is the classic histopathology of sarcoidosis [1,24] but there exists a spectrum of histology. Upon review of autopsy cases of cardiac sarcoidosis, granulomatous lesions were found in most of cases (108 patients) but in some cases (5 patients) myocardial scarring was a dominant lesion [18]. Histologic features were variable and the spectrum was divided into four types: exudative type, granuloma type, combined type, and fibrotic type (Table 3) [25]. Some extreme examples presented as cases without granuloma in any internal organs at autopsy, which had several years history of cardiac arrhythmia, and where the initial histological diagnosis was sarcoidosis in the lymph node [18]. Our cases with very small granuloma-like lesions on endomyocardial biopsy were interpreted as myocarditis or ignored instead of being interpreted as granulomas. Each granuloma was composed of 5–10 cells, and giant cells were not found.

A micro-granuloma is a type of small granuloma or granuloma-like lesion. The micro-granuloma was described in a medical dictionary as “a term of art referring to an aggregate of less than 25 epithelioid histiocytes” [26] and is associated with Crohn’s disease [27]. The micro-granuloma in our new definition was smaller than that of the dictionary. We further suggested the increase in scattered histiocytes as a variant of a lesion with similar significance of granuloma in cardiac sarcoidosis. A recent study revealed that the presence of CD3-, CD68-, and CD163-positive cells in endomyocardial biopsies of patients with dilated cardiomyopathy was associated with cardiac fibrosis and poor clinical outcomes [28]. The increase in CD68-positive cells may be an indicator of active myocardial inflammatory conditions, including sarcoidosis.

It is well documented that the histologic features of sarcoid granuloma are resolved by corticosteroid treatment [29,30] and spontaneous regression was observed in one-third of pulmonary sarcoidosis [31]. The case of cardiac sarcoidosis without granuloma at autopsy was interpreted as a result of steroid treatment in a previous report [18]. As is shown in the variable features of granulomas in the cardiac sarcoidosis on autopsied heart (Supplementary Fig. S7), presence of giant cells and histiocytes varies in the lesions and they will resolve to fibrotic lesions. Therefore, we would interpret the stromal micro-granuloma-like lesions as a healed stage of collection of scattered histiocytes.

The significance of confluent scars in the myocardium is also debatable. We agree that they are large replacement fibrosis [32] but they are different from the large scars of myocardial infarct by their distribution related to the coronary arterial supply. It is not always possible to obtain a large specimen on endomyocardial biopsy to find confluent fibrosis but it was possible in some cases. Macroscopic classification of myocardial lesions was described in three classes: spotty pattern, conglomerate band-like pattern, and dendritic pattern (Table 3) [25]. The detection of such patchy fibrosis may indicate the pathologic substrates of an apparent conduction delay of the electrical impulse and fractionation due to asynchronous activation in different tracts [32]. We therefore interpret large scars as probable evidence for cardiac sarcoidosis.

The presence of mature fat or “fibro-fatty replacement of myocardium” in endomyocardial biopsies is a hallmark of arrhythmogenic right ventricular cardiomyopathy [33-35]. However, the presence of mature epicardial fat in the endomyocardial biopsy is common. In such samples, we observed completely normal fatty tissue with direct attachment to the normal myocardium (Fig. 4D). Careful observation of fatty tissue in our endomyocardial biopsies revealed some different microscopic features, including fatty tissue associated with fibrosis and histiocytes. These fat cells often had small or variable cytoplasmic contours. We interpreted these as recently transformed fatty changes at the endocardial zone, or deep from the pericardium. It is not easy to differentiate these post-inflammatory fatty changes from those seen in the old myocardial infarcts. Fatty changes alone are not significant, but if fatty change is associated with young scars and histiocytes, it may be a clue to sarcoidosis.

When we diagnose the resected heart for cardiac transplantation or autopsy, we should evaluate the patient as a whole. We should include any previous pathologic processes in our final diagnosis. We would interpret hearts without definitive granuloma as a case of cardiac sarcoidosis if there is presence of micro-granulomas and macroscopic distribution of the lesion. The macroscopic distribution of cardiac lesions in our cases matches very well with the radiologic findings. There were scattered macroscopic fibrotic scars in the ventricular septum, free wall, sub-epicardium, and papillary muscles without any matching distribution in the coronary arterial supply [10,18,26]. This random pattern was also present in the autopsied heart with classical histologic features of sarcoidosis. If radiologic diagnosis of sarcoidosis is based on the macroscopic distribution of the lesion, the same feature of the resected heart can form the pathologic basis of cardiac sarcoidosis when we diagnose cardiac sarcoidosis for whole heart specimen. The macroscopic distribution of the granulomas was described as patchy and involving the ventricular septum and the subepicardium [36,37]. These features are confirmed by radiologic studies [38-40].

Differential diagnosis of cardiac sarcoidosis from the pathologists’ view was reviewed [1,24]. When we observe granulomas, we suspect sarcoidosis, giant cell myocarditis, and other granulomatous myocardial lesions, including those of infectious origin [20,21]. It may be necessary to broadly categorize these cases so that they are all classified into “granulomatous myocarditis.” if any specific causes are detected. In our experience with cases involving clinically suspected cardiac sarcoidosis, the pathologists are asked if the case is compatible with cardiac sarcoidosis, and to carefully examine the pathologic specimens to find any suggestive features and any small micro-granulomas. This attitude will support cardiologists in considering cardiac sarcoidosis to ensure they do not miss the chance to start optimal treatment. There are several different patterns of myocardial inflammatory lesions [1,41]. In general, viral myocarditis involves the heart as a diffuse lesion. The lesion may involve some parts more severely, but the margin of the inflammatory lesion is indistinct. Rheumatic myocarditis also has distinct features of involvement of the valve, pericardium, and perivascular interstitium. Infectious myocarditis involves only the focus of involvement. Tuberculous lesions involve predominantly pericardium first, and the myocardial lesions are an extension of pericarditis. For sarcoidosis, nodular and mass-like involvement is characteristic and the intervening myocardium between granulomata is generally spared. It has to be emphasized however that the end-stage fibrotic lesion of cardiac sarcoidosis will show different shape. We value the endomyocardial biopsy to exclude other differential diagnoses from sarcoidosis in the absence of granulomatous inflammation.

Explant hearts for transplantation have similar concerns. When cardiac sarcoidosis is clinically diagnosed, pathologists will check for micro-granuloma. It is worthwhile to remind pathologists that granulomas may be indistinct in cardiac sarcoidosis after treatment. High suspicion for cardiac sarcoidosis is necessary in patients with end-stage heart failure with pacemaker or implantable cardiac defibrillator [42]. Cardiologists and surgeons may incorrectly diagnose dilated cardiomyopathy or ischemic cardiomyopathy when the case was not associated with rhythm disturbance. However, involvement of the conduction system is not the rule in cardiac sarcoidosis [1,18,24,25]. The pathologists’ suspicion for cardiac sarcoidosis will require a search to find a granuloma or a micro-granuloma to confirm the diagnosis. It is also important for pathologists to examine the macroscopic morphology of the explant heart to find large confluent scars and fatty changes in the myocardium in both subendocardium and subepicardium.

Supplementary Materials

Notes

Ethics Statement

The study plan of this research was reviewed by the Seoul National University College of Medicine/Seoul National University Hospital (IRB NO. H-1802-050-921 and RESEARCH TITLE: Indirect pathological indicators for cardiac sarcoidosis on endomyocardial biopsy or the explant heart for transplantation). Review comments was: Since the risk of research is minimal, it is for expedited review, and the statement of reason for waiver of informed consent is reasonable. According to IRB Approval Criteria, the IRB approves the research. Further details of the rationale for this waiver was that this research was based on retrospective review of medical record and pathology slides, and anonymized case analysis. This analysis did not alter management of patients. See Attachment D: Informed consent and waiver of consent (https://www.hhs.gov/ohrp/sachrp-committee/recommendations/2013-january-10-letter-attachment-d/index.html).

Author contributions

Conceptualization: MJC, JWS, SO. Data curation: MJC, JWS, SO, EAP, SHL, MYK, JYP. Formal analysis: MJC, JWS, SO, EAP, SHL. Funding acquisition: JWS, SO, SHL. Investigation: MJC, JWS, SO, EAP, SHL, MYK, JYP. Methodology: MJC, JWS, SO, EAP, SHL, MYK, JYP. Project administration: JWS, SO. Resources: MJC, JWS, SO, EAP, SHL, MYK, JYP. Super vision: JWS, SO. Visualization: MJC, JWS. Writing—original draft: MJC, JWS. Writing—review & editing: MJC, JWS, SO, EAP, SHL.

Conflicts of Interest

The authors declare that they have no potential conflicts of interest.

Funding Statement

No funding to declare.

Acknowledgments

Fig. 1.

Micro-granuloma on the endomyocardial biopsy. (A) Endomyocardial biopsy at 2 years prior to the transplantation of case 1-1 shows confluent fibrosis with edematous stroma. Three foci of infiltration of histiocytes and lymphocytes (arrow) are seen at the margin of fibrosis which is the interface between the fibrosis and myocardium. (B) CD68 staining of the same specimen showing histiocytic infiltration at the micro-granulomas (arrow). (C) Endomyocardial biopsy of case 1-3 shows a micro-granuloma (arrow) of 15 cells in the fibrotic zone. (D) CD68 immunostaining of endomyocardial biopsy of case 1-3 shows positive staining (arrow) on histiocytic marker.

Fig. 2.

Micro-granuloma in an explant heart. (A) Magnification of the ventricular myocardium of explant heart in case 1-2 reveals interstitial fibrosis and a few small granulomas. (B) Immunohistochemical staining on CD68 in the same area shows a small nodular collection of histiocytes or zone of scattered histiocytes.

Fig. 3.

Histiocytic infiltration, confluent fibrosis and fatty change. (A) Confluent fibrosis, associated fatty change within the fibrosis in case 2-1. (B) CD68 staining in the same area shows increased histiocytes (arrows) scattered in the fibrous area. (C) Confluent fibrosis, associated fatty change within the fibrosis in case 3-1. (D) CD68 staining in the same area shows very rare or no increase of histiocytes (arrow) in the fibrous area.

Fig. 4.

Different types of fatty changes in endomyocardial biopsies. (A) Fatty infiltration in the background of confluent fibrosis (case 3-2). (B) Fatty tissue with variable sizes of adipocytes and adjacent myocardium also show post-inflammatory fibrosis (case 1-5). (C) Subendocardial deposition of fatty tissue. Slender fibrotic zone is visible at the margin of fatty area (case 3-5). (D) Fatty infiltration between the myocardial bundles. Adjacent myocardium is normal without fibrosis or inflammation (case 5-4).

Fig. 5.

Confluent fibrosis without associated fatty tissue. (A) Confluent fibrosis is not evident in a small biopsy but fibrosis bigger than five times the diameter of the myocardial cells was interpreted as a scar related to a granuloma (case 4-2). (B) Broad scar at the endocardial zone. Some adipocyte-like spaces were found but the scattered individual spaces were not interpreted as fatty change (Case 4-3).

Fig. 6.

A short-axis sectional view of the first transplant heart with sarcoidosis and fibrosis. (A) A short-axis sectional view of the heart shows multifocal confluent fibrosis involving both ventricles. The right ventricular thinning and dilatation are prominent. Coronary arteries and cardiac veins are filled with red and blue silicone rubber cast. (B) Histotopographic mapping of a short-axis plane of the heart by Masson’s trichrome staining reveals prominent fibrosis (in blue color) in the right ventricular free wall and patchy fibrosis in the ventricular septum and left ventricle.

Fig. 7.

The second transplant heart with sarcoidosis and hypertrophied ventricles. (A) Sectional view of the heart shows multifocal confluent fibrosis involving predominantly left ventricle and the ventricular septum. Epicardial fatty tissue is prominent in the right ventricle but the myocardium is not much involved. (B) Histotopographic mapping of a short-axis plane of the heart by Masson’s trichrome staining reveals prominent fibrosis (in blue color) in the interventricular septum and the left ventricular free wall. Distribution of the fibrosis is prominent but not limited to the subepicardial zone.

Fig. 8.

The third transplant heart mimicking sarcoidosis on microscopy but not likely of sarcoidosis on macroscopic view. (A) The left ventricle is dilated and hypertrophied. Multifocal and diffuse fibrosis was noted on gross examination. Fatty infiltration was prominent at the anterior part of the ventricular septum and mid-septal fibrosis was evident. (B) Fatty change associated with myocardial fibrosis was seen at the anterior part of the ventricular septum. (C) Low magnification of the mid-septal fibrosis (Masson’s trichrome stain).

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